Ways of increasing the efficiency of immunodiagnostic bacterial preparations
Abstract
Indirect haemagglutination (IHA) test remains as before in an arsenal of researchersґs diagnostic tests both in medical and veterinary fields owing to fortunate combination of such factors as high specificity and savings on costs. Nevertheless diagnostic antigens currently using in laboratories, unfortunately, are characterized by low sensitivity and a short storage life. In the article some methods for development of IHA immunodiagnostic test-systems in two main directions as a pairing component using to enhance adsorption activity of a carrier (erythrocyte) and to increase immunodiagnostic antigens storage life and also highly active antigen selecting for erythrocytes sensitization to improve diagnostic antigen sensitivity are offered and considered. Test data of diagnostic test-systems with the water-soluble polymer poly-1-vinyl imidazol as a conjugate in comparison with generally accepted conjugate, chromium chloride, are analyzed. Antigen activity of microbal cell different particles at animals’ immunization and in structure diagnostic test-systems itself is assessed. Advantages of diagnostic test-systems new components application are proved too. The applied technology of preparing antigenic erythrocyte diagnostic systems promotes an improvement of their qualitative characteristics such as specificity, sensitivity, storage life, results reproducibility.
Key words
ReferencesSerdyuk L.V. – candidate of biological sciences, researcher of the laboratory of microecology,
e-mail: radugarose@yandex.ru
Leshchuk S.I. – doctor of biological sciences, leading researcher of the laboratory of microecology,
e-mail: leschuk.swet@yandex.ru
Popkovа S.M. – doctor of biological sciences, head of laboratory of microecology, e-mail: smpopkova@gmail.com
Yurinova G.V. – candidate of biological sciences, associate professor of the chair for physical and chemical biology, e-mail: yurinova@yandex.ru
Rakova E.B. – candidate of biological sciences, researcher of the laboratory of microecology,
e-mail: lenova_@mail.ru
