Ultrastructural study of endometrial receptivity in pregravid treatment conditions at recurrent miscarriage

OBJECTIVE: To study the dynamics of ultrastructural changes of endometrial epithelial cells at the result of pregravid treatment of the recurrent miscarriage. MATERIAL AND METHODS: 63 samples of endometrial biopsies of women in reproductive age with a luteal phase unsufficiency. Light and electron microscopy. RESULTS AND DISCUSSION: It has been revealed that pregravid cyclic hormone therapy (1 mg of 17?-estradiol and 20 mg of dydrogesterone) in comparisson with monotherapy by 20 mg of dydrogesterone during 3 months induced the intracellular regeneration and the implementation of the secretory phenotype of endometrial epithelial cells corresponding to the receptivity status with an "implantation open window".

1. Milovanov A.P., Gerasomova O.P. Clinical-morphological control of the pregravid treatment efficiency in women after non-developing pregnancies // Akusherstvo i ginekologiya. 2013. (5). 87-90. (in Russian)
2. Nepomnyashchikh G.I. Bronchial biopsy: Morphogenesis of general pathological processes in lungs. Moscow, 2005. 384 p. (in Russian)
3. Radzinskiy V.E., Dimitrova V.I., Mayskova I.U. Non-developing pregnancy. Manual. Moscow: GEOTAR-Media, 2009. 200 p. (in Russian)
4. Sidelnikova V.M. Habitual pregnancy loss. Moscow: Triada-X, 2005. 303 p. (in Russian)
5. Sukhikh G.T., Vanko L.V. Immunology of pregnancy. Moscow, 2003. 400 p. (in Russian)
6. Khmelnitskiy O.K. Pathomorphological diagnostics of gynecological diseases. Saint-Petersburg: SOTIS, 1994. 480 p. (in Russian)
7. Tsellarius U.G., Semenova L.A., Nepomnyashchikh L.M. et al. Favorites fundamental problems of myocardial histopathology // Byulleten' Sibirskogo otdeleniya Akademii meditsinskikh nauk Soyuza Sovetskikh Sotsialisticheskikh Respublik. 1982. (3). 22-29. (in Russian)
8. Badawy A.M., El-Gharib M.E., Zalata K. Should endometrial biopsy be a routine investigation for recurrent early-pregnancy loss? // Gynaecol. Endosc. 2002. 11. (6). 397-399.
9. Ford H.B., Schust D.J. Recurrent pregnancy loss: etiology, diagnosis, and therapy // Rev. Obstet. Gynecol. 2009. 2. (2). 76-83.
10. Lea R.G., Oliver S.S. Immunoendocrine aspects of endometrial function and implantation // Reproduction. 2007. 134. (3). 389-404.
11. Prado-Drayer A., Teppa J., Sanchez P., Ca­mejo M.I. Immunophenotype of peripheral T-lympho­cytes, NK cells and expression of CD69 activation marker in patients with recurrent spontaneous abortions during the mid-luteal phase // Am. J. Reprod. Immunol. 2008. 60. (1). 66-74.
12. Szekeres-Bartho J., Balasch J. Progestagen therapy for recurrent miscarriage // Hum. Reprod. Update. 2008. 14. (1). 27-35.

Marinkin I.O. - doctor of medical sciences, professor, head of the chair for obstetrics and gynecology, rector
Nepomnyashchikh D.L. - doctor of medical sciences, professor, head of the laboratory for clinical morphology, deputy director on scientific work
Kuleshov V.M. - doctor of medical sciences, professor of the chair for obstetrics and gynecology
Ilizarova N.A. - doctor of medical sciences, associate professor of the chair for obstetrics and gynecology
Semchenko T.O. - post graduate student of the chair for obstetrics and gynecology
Aidagulova S.V. - doctor of biological sciences, professor, head of the laboratory for cellular biology and fundamental basis of reproduction, e-mail: a_sv@ngs.ru