Pathological changes and expression of vascular endothelial growth factor in the placenta in inherited thrombophilia

Summary. The aim was to study the pathological changes in the placenta and VEGF expression in the cells of acroteric villus in case of inherited thrombophilia. For the study, samples were taken from 37 placentas of women, divided into 3 groups: the group 1 comprised 13 pregnant women with verified inherited thrombophilia (35.3 %); the group 2 included 14 pregnant women with inherited thrombophilia and signs of inflammatory manifestations in the placenta (basal deciduitis, intervillesitis, placentitis) (37.84 %); the third (control) group included 10 pregnant women with normal pregnancy. Morphological manifestations of chronic placental insufficiency in the form of pathological immaturity with a predominance of differentiated intermediate villi and dissociated maturation cotyledons with a substantial reduction of the specialized terminal villi have been revealed in placenta at hereditary thrombophilia. Decrease in the number of capillaries in the terminal villi was due to reduced expression of vascular endothelial growth factor in cells of terminal villi.

1. Barkagan Z.S., Momot A.P. Diagnosis and treatment of hemostatic disorders controlled . Moscow, 2001 . 296 p. (in Russian)
2. Bicadze V.O. Makatsaria A.D., Hizroeva D.H. et al. Thrombophilia as a key link in the pathogenesis of complications of pregnancy // Practice of medicine. 2012. 9. 24-31. (in Russian)
3. Glantz S. Biostatistics. Moscow, 1999. 459 p. (in Russian)
4. Dolzenko T.A. Milovanov A.P., Davtyan E.L. Morphological diagnosis and pathogenesis of developing pregnancy in antiphospholipid syndrom // Arkhiv patologii. 2005. (2). 9-3. (in Russian)
5. Zhukova V.A., Nadeev A.P., Drobinskaya A.N. et al. Morphological and immunohistochemical study of utero- placental area in thrombophilia // Vestnik Novosibirskogo gosudarstvennogo universiteta. Seriya Biologiya, klinicheskaya meditsina. 2012. 10. (2). 151-159. (in Russian)
6. Makatsaria A.D. Bicadze V.O. Thrombophilic state in obstetric practice. Moscow, 2001. 219-285. (in Russian)
7. Milovanov A.P. Pathology mother - placenta - fetus. Moscow, 1999. 447 p. (in Russian)
8. Milovanov A.P., Sidorov I.S., Solonitsyn A.N., Borovkov E.I. Immunohistochemical assessment of the distribution of vascular endothelial growth factor in the placenta, placental bed of the uterus during normal pregnancy and in women with preeclampsia // Arkhiv patologii. 2008. (3). 12-15. (in Russian)
9. Milovanov A.P., Serova O.F. Causes and differentiated treatment of early pregnancy loss. Moscow, 2011. 216 p. (in Russian)
10. Momot A.P. Taranenko I.A. Tsyvkina L.P. The evolution of ideas about thrombophilia and its role in human reproduction problems // Akusherstvo i ginekologiya. 2013. (2). 4-9. (in Russian)
11. Sarkisov D.S., Perov J.L. Microscopic technique. Moscow, 1996. 544 p. (in Russian)
12. Serov V.N., Pasman N.M., Sturov V.G., Drobinskaya A.N. Hereditary and acquired thrombophilic states in obstetric practice. Novosibirsk, 2011. 180 p. (in Russian)
13. Serov V.N., Tyutyunnik V.L., Mikhailov O.I. Modern views on the treatment of placental insufficiency // Russkiy meditsinskiy zhurnal. 2011. 3. 3-5. (in Russian)
14. Strizhakov A.N. Voloshchuk I.N., Timokhina E.V. et al. The clinical significance of thrombophilia in the development of apoptosis and proliferation in placental insufficiency // Voprosy ginekologii, akusherstva i perinatologii. 2010. 9. (4). 5-12. (in Russian)
15. Suchich G.T., Dobrokhotova J.E., Faizullin L.Z. Role of hyperhomocysteinemia in the genesis of developing pregnancy and miscarriage started // Russkiy meditsinskiy zhurnal. 2005. 13. (17). 1110-1113. (in Russian)
16. Trifonova E.A., Gabidulina T.V., Agarkova T.A. et al. Analysis of the role of hereditary thrombophilia in the development of complicated pregnancy // Fundamental'nye issledovaniya. 2012. (10). 337-344. (in Russian)
17. Baksu A., Taskin M., Goker N. et al. Plasma homocysteine in late pregnancies complicated with preeclampsia and in newborns //Am. J. Perinatol. 2006. 23. (1). 31-35.
18. Chou A.K., Hasieh S.C., Su Y.N. et al. Neonatal and pregnancy outcome in primare antiphospholipid syndrom a 10-year experience in one medical center // Pediatr. Neonatol. 2009. 50. (4). 143-146.
19. Costa S.L., Proctor L., Dood J.M. et al. Screening for placental insufficiency in high-risk pregnancies isearlier better // Placenta. 2008. 29. (12). 1034-1040.
20. Neerhof M.G., Thaete L.G. The fetal response to chronic placental insufficiency // Semin. Perinatol. 2008. 32. 201-205.
21. Pavlov O.V., Sheveleva T.S., Selkov S.A. In vitro expression of vascular endothelial growth factor and its receptors by placental macrophages. // Bull. Exp. Biol. Med. 2012. 153. (2). 222-225.
22. Shibuya M. Vascular endothelial growth factor and its receptor system: physiological functions in angiogenesis and pathological roles in various diseases // J. Biochem. 2013. 153. (1). 13-19.
23. Tabata T., Petitt M., Fang-Hoover J. et al. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model // Am. J. Pathol. 2012. 181. (5). 1540-1559.

Nadeev A.P. - doctor of medical sciences, professor of the department for pathology, e-mail: nadeevngma@mail.ru
Drobinskaya A.N. - candidate of medical sciences, deputy chief
Zhukova V.A. - candidate of medical sciences, assistant professor of the department for pathology
Karpov M.A. - candidate of medical sciences, assistant of the department for pathology
Travin M.A. - candidate of medical sciences, senior assistant professor of the department for pathology, e-mail: mtravin@mail.ru
Cherdantseva L.A. - candidate of medical sciences, senior researcher of the laboratories for pathology