Molecular mechanisms of hydrogen sulfide regulatory influence on cell cycling state progression

The aim of the study: identification of molecular mechanisms of hydrogen sulfide influence on cell cycle progression. Material and methods: Jurkat cells were the material of the study. Methods included flow cytometry, western blotting, RT-PCR. Results and discussion: The delay of G1 cell cycle phase connected with the decrease in content of phosphorylated form of retinoblastoma protein in 50 μM NaHS-treated cells. Phosphorylation of retinoblastoma protein takes place when cyclins activate cycline-dependent kinases. It has been shown that hydrogen sulfide in low concentration leads to decrease of cycline D1 level via decrease of respective gene expression.

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