Author Affiliations1FSBI «RI for Epidemiology and Microbiology named after G.P. Somov» of SB RAMS 690087, Vladivostok, Selskaya str., 1
2FSBI «RI for Epidemiology and Microbiology named after G.P. Somov» of SB RAMS 690087, Vladivostok, Selskaya str., 1; Far Eastern Federal University 690091, Vladivostok, Octyabrskaya str., 27
3FSBI «RI for Epidemiology and Microbiology named after G.P. Somov» of SB RAMS 690087, Vladivostok, Selskaya str., 1
4Far Eastern Federal University 690091, Vladivostok, Octyabrskaya str., 27
5FSBI «RI for Epidemiology and Microbiology named after G.P. Somov» of SB RAMS 690087, Vladivostok, Selskaya str., 1
The data on definition of the functional activity of the neutrophils infected by the Hantaviruses have been presented. The enzymes – 5’-nucleotidase, adenosine triphosphatase, lactate dehydrogenase, myeloperoxidase, cytochrome c oxidase and the cationic proteins have been studied. It has been revealed that the Hantavirus is capable to infect the neutrophils, has evident influence to their oxygen forming activity. Thus the tendency to anaerobic energy production pathway in the cells has been revealed, that agrees with the increase in the LDH и CCO activity. Whereas a low MPO activity in the neutrophils infected by the Hantavirus indicates the suppression of the cell protection reaction upon the virus introduction. The Hantavirus ability to adhere to the neutrophils surface and change their morphological and functional characteristics was confirmed by the scanning electron microscopy.
1. Kirilicheva G.B., Solovyeva M.S., Baturina I.G. et al. The sensitivity to mouse toxin and the level of 5-nucleotidase macrophages activity // Bulleten' experimental'noy biologii i meditsiny. 1992. 3. 296–297. (in Russian)
2. Kokryakov V.N. Essays on innate immunity. Saint-Petersburg: Nauka, 2006. (in Russian)
3. Plekhova N.G., Somova L.M., Drobot E.I. et al. Changes in the metabolic activity of macrophages under the influence of tick-borne encephalitis // Biochemistry (Moscow). 2007. 72 (2). 236–246. (in Russian)
4. Plekhova N.G., Lyapun I.N., Somova L.M. et.al. Reactivity of innate immunity cells in the pathogenesis of hantavirus infection // Byulleten' Vostochno-Sibirskogo nauchnogo tsentra Sibirskogo otdeleniya Rossiyskoy akademii meditsinskikh nauk. 2012. 85 (3). 296–300. (in Russian)
5. Rovensky Yu.A. Raster electronic microscopy of normal and tumor cells. M.: Meditsina, 1979. (in Russian)
6. Borregaard N., Cowland J.B. Granules of the human neutrophilic polymorphonuclear leukocyte // Blood. 1997. 89. 3503–3521.
7. Kennedy A.D., DeLeo F.R. Neutrophil apoptosis and the resolution of infection // Immunol. Res. 2009. 43. 25–61.
8. Larochelle B., Flamand L., Gourde P. et al. Epstein-Barr virus infects and induces apoptosis in human neutrophils // Blood. 1998. 92. 291–299.
9. Sadeghi M., Eckerle I., Daniel V. et al. Cytokine expression during early and late phase of acute Puumala hantavirus infection // BMC Immunol. 2011. 12:65. doi:10.1186/1471-2172-12-65.
10. Salmen S., Montes H., Soyano A. et al. Mechanisms of neutrophil death in human immunodeficiency virus-infected patients: role of reactive oxygen species, caspases and map kinase pathways // Clin. Exp. Immunol. 2007. 150. 539–545.
11. Tesfaldet T., White M.R., Gantz D. et al. Human neutrophil defensins increase neutrophil uptake of influenza A virus and bacteria and modify virus-induced respiratory burst responses // J. Immunol. 2007. 178. 8046–8052.
12. Witko-Sarsat V., Rieu P., Descamps-Latscha L. et al. Neutrophils: molecules, functions and pathophysiological aspects // Lab. Invest. 2000. 80. 617–653.
13. Yrlid U., Jenkins C.D., MacPherson G.G. Relationships between distinct blood monocyte subsets and migrating intestinalё lymph dendritic cells in vivo under steady-state conditions // J. Immunol. 2006. 176. 4155–4162.
About Authors (Correspondence):
Lyapun I.N. – junior researcher of the laboratory of pathomorphology and electron microscopy, е-mail: irina-lyapun@list.ru
Plekhova N.G. – doctor of biological sciences, head of the laboratory of pathomorphology and electron microscopy, e-mail: pl_nat@hotmail.com
Kompanets G.G. – candidate of medical sciences, leading researcher of the laboratory of hemorrhagic fever with renal syndrome, e-mail: galkom@inbox.ru
Smirnov I.S. – postgraduate student of the laboratory interdisciplinary of electron microscopy and image processing, e-mail: smirnov@lemoi.phys.dvgu.ru
Somova L.M. – doctor of medical sciences, professor, chief researcher of the laboratory of pathomorphology and electron microscopy, e-mail: l_somova@mail.ru
