Thymic and spleens cellular structure of mice in dynamics of development of chronic
Author Affiliations1Institute of Clinical and Experimental Lymphology SB RAMS 630117, Novosibirsk, Timakov str., 4
2Institute of Clinical and Experimental Lymphology of SB RAMS 630117, Novosibirsk, Timakov str., 4
3Institute of Clinical Immunology of SB RAMS, 630099, Novosibirsk, Yadrintsevskaya str., 14
4Institute of Clinical Immunology of SB RAMS, 630099, Novosibirsk, Yadrintsevskaya str., 14
5Institute of Physiology SB RAMS 630117, Novosibirsk, Timakov str., 4
Abstract
RESEARCH OBJECTIVE. Studying of thymic and spleens cellular structure of mice at chronic "graft-versus-host" reaction. MATERIAL AND METHODS. Induction of chronic "graft-versus-host" reaction at mice was carried out by transfer to females of B6D2F1 of lymphoid cells of the parental DBA/2 line. Subpopulations of lymphocytes, proliferation and apoptosis were determined by a method of a flow cytometry with use of monoclonal antibodies and propidium iodide. RESULTS AND DISCUSSION. It is revealed that in dynamics of development of chronic "graft-versus-host" reaction interactions between the central and peripheral components of immunity are broken. The results allow considering the processes of lymphopoiesis in thymus as a target for correction of autoimmune diseases and chronic "graft-versus-host" reaction.
Key words
References1. Kolesnikova O.P., Mirskova A.N., Adamovich S.N. et al. Screening of immunoactive and antitumor properties of triethanolamine complex with salts of biotrace elements // Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk. 2009. 140. (6). 73-80. (in Russian)
2. Kudaeva O. T. Th1/Th2-polyarization as the epigenetic mechanism of formation of different options of an immunopathology at reaction "graft-versus-host": abstract of thesis…doctor of medical sciences. Novosibirsk, 2005. (in Russian)
3. Kudaeva O. T., Kolesnikova O.P. Pathophysiological mechanisms of immune disorders and balance of pro- and anti-inflammatory cytokines. GVHR model // System of cytokines. Theoretical and clinical aspects. Ed. V.A. Kozlov, S.V. Sennikov. Novosibirsk: Nauka, 2004. 255-268. (in Russian)
4. Limonov V.L., Shurlygina A.V., Robinson M.V. et al. The use of the derivative of indoliltiocarbonic acid (compound VL-11-02) as immunocorrector with the help of immunocomplex glomerulo-nephritis model // Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk. 2005. 116. (2). 50-55. (in Russian)
5. Trufakin V.A. Immunomorphological aspects of autoimmune processes. Novosibirsk: Nauka, 1983. 178 p. (in Russian)
6. Appleby P., Webber D.G., Bowen J.G. Murine chronic graft-versus-host disease as model of systemic lupus erythematosus: Effect of immunosuppressive drugs on disease development // Clin. Exp. Immunol. 1989. 78. (3). 449-453.
7. Chen X., Vodanovic-Jankovic S., Johnson B. et al. Absence of regulatory T-cell control of TH1 and TH17 cells is responsible for the autoimmune-mediated pathology in chronic graft-versus-host disease // Blood. 2007. 110. (10). 3804-3813.
8. Chu Y.W., Gress R.E. Murine models of chronic graft-versus-host disease: insights and unresolved issues // Biol. Blood Marrow Transplant. 2008. 14. (4). 365-378.
9. Diamond B. Autoimmunity // Immunol. Rev. 2005. 204. 5-8.
10. Ercolini A.M., Miller S.D. The role of infections in autoimmune disease // Clin. Exp. Immunol. 2009. 155. (1). 1-15.
11. Fairweather D., Frisancho-Kiss S., Rose N.R. Sex differences in autoimmune disease from a pathological perspective // Am. J. Pathol. 2008. 173. (3). 600-609.
12. Invernizzi P., Lleo A., Podda M. Interpreting serological tests in diagnosing autoimmune liver diseases // Semin. Liver. Dis. 2007. 27. (2). 161-172.
13. Kapina M.A., Shepelkova G.S., Mischenko V.V. et al. CD27low CD4 T lymphocytes that accumulate in the mouse lungs during mycobacterial infection differentiate from CD27high precursors in situ, produce IFN-gamma, and protect the host against tuberculosis infection // J. Immunol. 2007. 178. (2). 976-985.
14. Katsiari C.G., Kyttaris V.C., Juang Y.T., Tsokos G.C. Protein phosphatase 2A is a negative regulator of IL-2 production in patients with systemic lupus erythematosus // J. Clin. Invest. 2005. 115. (11). 3193-3204.
15. Murphy W.J. Revisiting graft-versus-host disease models of autoimmunity: new insights in immune regulatory processes // Ibid. 2000. 106. (6). 745-747.
16. Paust S., Cantor H. Regulatory T cells and autoimmune disease // Immunol. Rev. 2005. 204. 195-207.
17. Sakoda Y., Hashimoto D., Asakura S. et al. Donor-derived thymic-dependent T cells cause chronic graft-versus-host disease // Blood. 2007. 109. (4). 1756-1764.
18. Schiцtt A., Lindstedt M., Johansson-Lindbom B. et al. CD27- CD4+ memory T cells define a differentiated memory population at both the functional and transcriptional levels // Immunology. 2004. 113. (3). 363-370.
19. Slayback D.L., Dobkins J.A., Harper J.M., Allen R.D. Genetic factors influencing the development of chronic graft-versus-host disease in a murine model // Bone Marrow Transplant. 2000. 26. (9). 931-938.
20. Via C.S., Shearer G.M. Defective in vitro IL-2 production in lupus is an early but secondary event paralleling disease activity: evidence from the murine parent-into-F1 model supports staging of IL-2 defects in human lupus // Autoimmunity. 2010. 43. (1). 23-31.
About Authors (Correspondence):
Shurlygina A.V. - doctor of medical sciences, professor, head of the laboratory of immunomorphology, e-mail: anna_v_s@mail.ru
Melnikova E.V. - researcher of the laboratory of immunomorphology, e-mail: gennyma@gmail.com
Kolesnikova O.P. - doctor of medical sciences, head of the laboratory of experimental immunotherapy, e-mail: iscreen2001@mail.ru
Goiman E.V. - candidate of medical sciences, researcher of the laboratory of experimental immunotherapy, e-mail: L.Goiman@mail.ru
Trufakin V.A. - doctor of medical sciences, professor, academician of RAMS, head of the laboratory of chronophysiology, e-mail: trufakin@physiol.ru
Full Text
