№ 4 / 2013 / 5-11

Peculiarities of lipid and glucose metabolism in hypertensive rats of strain ISIAH

Author: Kovshik G.G.¹, Khrapova M.V.², Dushkin M.I.³

Abstract

The work was aimed at the comparative study of serum lipid profile, glucose, corticosterone, TNF-a, IL6, liver PPAR-a and -g DNA-binding activity and blood pressure levels in six-monthly hypertensive rats of ISIAH strain and normotensive rats of WAG strain under normal conditions and by high-fat diet, injection of E. coli lipopolysaccharide (LPS), bezafibrate, and constant light have been studied. It was found that most studied indices in intact ISIAN rats are distinguished from WAG rats significantly. In contrast to WAG rats, high-fat diet did not lead to sign development of metabolic syndrome in ISIAH rats which however were more sensitive to LPS and bezafibrate exposure and less responsive to constant light. Thus, the ISIAH rats may be a genetic model to uncover novel mechanisms of resistance to metabolic syndrome, hypersensitivity to inflammatory stimuli and adaptation resources of organism.

Key words

PPAR, bacterial endotoxin, hypertensive ISIAN rats, desynchronosis, high-fad diet, corticosterone., lipid and glucose metabolism

References

1. Buzueva I.I., Filyushina E.E., Shmerling M.D. et al. Structural characteristics of the adrenal cortex from rats with stress-induced hereditary arterial hypertension in the late postnatal ontogenesis // Byulleten' eksperimental'noi biologii i meditsiny. 2012. 153. (9). 380-383. (in Russian)

2. Markel A.L., Kalashnikova E.V., Goryakin S.V. et al. Functional activity of the sympathoadrenal system in hypertensive NISAG rats // Bull. Exp. Biol. Med. 2006. 141. 244–247. (in Russian)

3. Markel A.L., Shishkina G.T. Genetic correlation between the arterial pressure response during emotional stress and the alpha1-adrenoreceptor concentration in brain regions // Genetika. 1992. 28. (11). 130-133. (in Russian)

4. Pivovarova E.N., Dushkin M.I., Perepechaeva M.L. et al. All signs of metabolic syndrome in the hypertensive ISIAH rats are associated with increased activity of transcription factors PPAR, LXR, PXR, and CAR in the liver // Biomeditsinskaya khimiya. 2011. 57. (4). 480–489. (in Russian)

5. Tinnikov A.A., Bazhan N.M. Determination of glucocorticoids in blood and adrenal gland cultures by competitive protein binding without preliminary extraction // Laboratornoye delo. 1984. 12. (7). 709-713. (in Russian)

6. Khvorostova Yu.V., Goriakin S.V., Pertrova G.V. et al. Function of hypothalamic-pituitary-adrenocortical system in hypertensive rats of ISIAH strain // Rossiyskiy fiziologicheskiy zhurnal imeni Ivana Mikhaylovicha Sechenova. 2002. 88. (11). 1423-1432. (in Russian)

7. Han J., Jiang D-M., Du C-Q. Alteration of enzyme expressions in mevalonate pathway: possible role for cardiovascular remodeling in spontaneously hypertensive rats // Circ. J. 2011. 75. (6). 1409–1417.

8. Hattori A., Mawatari K., Tsuzuki S. et al. Beta-adrenergic-AMPK pathway phosphorylates acetyl-CoA carboxylase in a high-epinephrine rat model, SPORTS // Obesity (Silver Spring). 2010. 18. (1). 48–54.

9. Kitts D.D., Yuan Y.V., Godin D.V. Plasma and lipoprotein lipid composition and hepatic antioxidant status in spontaneously hypertensive (SHR) and normotensive (WKY) rats // Can. J. Pharmacol. 1998. 76. (2). 202–209.

10. Liu D., Ji L., Zhang D. et al. Nonenzymatic glycation of high-density lipoprotein impairs its anti-inflammatory effects in innate immunity // Diabetes Metab. Res. Rev. 2012. 28. (2). 186–195.

11. Markel A.L., Redina O.E., Gelinsky M.A. et al. Neuroendocrine profiling in inherited stress-induced arterial hypertension rat strain with stress-sensitive arterial hypertension // J. Endodocrinol. 2007. 195. (3). 439–450.

12. Mormede P., Foury A., Barat P. et al. Molecular genetics of hypothalamic-pituitary-adrenal axis activity and function // Ann. N. Y. Acad. Sci. 2011. 1220. 127–136.

13. Van den Brandt J., Kovacs P., Kloting I. Metabolic features in disease-resistant as well as in spontaneously hypertensive rats and newly established obese Wistar Ottawa Karlsburg inbred rats // Int. J. Obes. Relat. Metab. Disord. 2000. 24. (12). 1618–1622.

About Authors (Correspondence):

Kovshik G.G. – postgraduate student, e-mail: midushkin@soramn.ru

Khrapova M.V. – candidate of biological sciences, senior researcher, e-mail: marina.chrapova@gmail.com

Dushkin M.I. – doctor of medical sciences, professor, head of laboratory for molecular and cellular mechanisms, e-mail: midushkin@soramn.ru

Full Text

1-4-2013.pdf

Received: 02/02/2015