The study of the response of rat’s ocular blood flow to the administration of vascular endothelial growth factor by ultrasonic Doppler methods
Author Affiliations1Moscow Helmholtz Research Institute of Eye Diseases, 105062, Moscow, Sadovaya-Chernogryazskaya str., 14/19
2Moscow Helmholtz Research Institute of Eye Diseases of Minzdrav of Russia, 105062, Moscow, Sadovaya-Chernogryazskaya str., 14/19
3Moscow Helmholtz Research Institute of Eye Diseases of Minzdrav of Russia, 105062, Moscow, Sadovaya-Chernogryazskaya str., 14/19
4Moscow Helmholtz Research Institute of Eye Diseases of Minzdrav of Russia, 105062, Moscow, Sadovaya-Chernogryazskaya str., 14/19
5Moscow Helmholtz Research Institute of Eye Diseases of Minzdrav of Russia, 105062, Moscow, Sadovaya-Chernogryazskaya str., 14/19
Abstract
The ocular blood flow in rat’s eyes in vivo has been studied with the use of modern ultrasonic methods and a scheme of the flow has been presented. Marked similarities in the structure of ocular blood vessels in rats and humans are noted. The influence of intravitreal administration of the vascular endothelial growth factor on ocular blood flow has been studied by means of an experimental model of ocular neovascularization. The initial brief increase in the ocular blood flow as a reaction to the development of ischemia was followed by the normalization or reduction of the parameters of the blood flow.
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References1. Kiseleva T.N. Color Doppler imaging in ophthalmology // Vestnik oftal'mologii. 2001. (6). 50–52. (in Russian)
2. Kiseleva T.N., Kravchuk E.A Нemodynamic characteristics of rabbit eye and orbit studied by modern ultrasonic methods // Refraktsionnaya khirurgiya i oftal'mologiya. 2005. (4). 53–56. (in Russian)
3. Patent 2488891 RF. Method for simulating anterior segment neovascularization in rats / Neroev V.V., Khoroshilova-Maslova I.P., Ryabina M.V. et al.; published 27.07.13. (in Russian)
4. Hammes H.P., Porta M. Experimental approaches to diabetic retinopathy // Front. Diabetes. Basel, Karger, 2010. 42–600.
5. Holinski S., Knebel F., Heinze G. et al. Noninvasive monitoring of cardiac function in a chronic ischemic heart failure model in the rat: assessment with tissue Doppler and non-Doppler 2D strain echocardiography // Cardiovasc. Ultrasound. 2011. 26. (9). ID PMC3118319.
6. Kaiser H., Flammer J., Hendricson Ph. Ocular blood flow. Basel, Karger, 1996. 226 p.
7. Sugiyama K.K., Gu Z.B., Kawase C. et al. Optic nerve and peripapillary choroidal microvasculature of the rat eye // Invest. Ophthalmol. 1999. 35. 3897–3904.
8. Sun J., Lindvere L., van Raaij M.E. et al. In vivo imaging of cerebral hemodynamics using high-frequency micro-ultrasound // Cold Spring Harb. Protoc. 2010. 9. 5495.
9. Ypsilantis P., Deftereos S., Prassopoulos P., Simopoulos C. Ultrasonographic diagnosis of pregnancy in rats // J. Am. Assoc. Lab. Anim. Sci. 2009. 48. (6). 734–739.
About Authors (Correspondence):
Neroev V.V. – doctor of medical sciences, professor, head of retinal pathology department
Kiseleva T.N. – doctor of medical sciences, professor, head of ultrasound department
Ryabina M.V. – candidate of medical sciences, senior researcher of retinal pathology department
Okhotsimskaya T.D. – candidate of medical sciences, physician of retinal pathology department, e-mail: tata123@inbox.ru
Zolotych O.V. – candidate of medical sciences, physician of retinal pathology department
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