№ 2 / 2015 / 31-36

The involvement of reactive oxygen species and redox-sensitive signaling system Keap1/Nrf2/ARE in the development of granulomatous inflammation

Author: Menshchikova E.B.¹, Zenkov N.K², Chechushkov A.V.³, Kozhin P.M.⁴, Cherdantseva L.A.⁵, Sharkova T.V.⁶, Potapova O.V.⁷, Lyubimov G.Yu.⁸, Lyubimova G.A.⁹, Yagunov S.E.¹⁰

Author Affiliations

Abstract

The effect of phenolic antioxidant 3-(3'-tert-butyl-4'-hydroxyphenyl) propylthiosulphonate sodium (TS-13), which is an activator of redox-sensitive system Keap1/Nrf2/ARE, is investigated in the model of BCG vaccine intravenous injection. Granulomas of presumably macrophagic type were formed in liver of BALB/c mice at day 30 after infection, activity of free-radical oxidation processes in liver enhanced too and directly correlated with the granuloma numerical density and diameter. Activity of free-radical oxidation processes as well as granuloma formation significantly decreased in animals which received TS-13 (100 mg/kg body weight) in drinking water, whereas generation of reactive oxygen species by peritoneal exudate phagocyte increased. The data indicate the involvement of Keap1/Nrf2/ARE system in granulomogenesis and the dichotomy of the body reaction to it.

Key words

TS-13, reactive oxygen species, granulomatous inflammation, macrophage, Keap1/Nrf2/ARE system

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About Authors (Correspondence):

Menshchikova E.B. – doctor of medical sciences, head of the laboratory, e-mail: lemen@centercem.ru

Zenkov N.K. – doctor of biological sciences, leading researcher, e-mail: lemen@centercem.ru

Chechushkov A.V. – researcher, e-mail: achechushkov@gmail.com

Kozhin P.M. – researcher, e-mail: kozhinpm@gmail.com

Cherdantseva L.A. – candidate of medical sciences, leading researcher, e-mail: cherdanceff@yandex.ru

Sharkova T.V. – candidate of biological sciences, leading researcher, e-mail: tasharkova@yandex.ru

Potapova O.V. – doctor of medical sciences, head of the laboratory, e-mail: potapova@centercem.ru

Lyubimov G.Yu. – candidate of medical sciences, researcher, e-mail: glubimov@rambler.ru

Lyubimova G.A. – technician, e-mail: glubimov@rambler.ru

Yagunov S.E. – postgraduate student, e-mail: semenyagunov@gmail.com

Full Text

5-2-2015.pdf

Received: 17/04/2015