Investigation of degenerative processes in the femoral head of rats with prolonged alcohol intoxication
Author Affiliations1Institute of Chemical Biology and Fundamental Medicine of SB RAS, 630090, Novosibirsk, Akademik Lavrent’ev av., 8
2N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of SB RAS, 630090, Novosibirsk, Academik Lavrentev av., 9
3Institute of Chemical Biology and Fundamental Medicine of SB RAS, 630090, Novosibirsk, Akademik Lavrent’ev av., 8
4N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of SB RAS, 630090, Novosibirsk, Academik Lavrentev av., 9
5N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of SB RAS, 630090, Novosibirsk, Academik Lavrentev av., 9
Abstract
The research objective. The study of the effect of chronic alcohol intoxication on the development of degenerative processes in the femoral head of rats. Material and Methods. Chronic alcohol intoxication was modeled on male rats weighing 200–220g. For studies the rats were randomly assigned to groups: I and II groups received daily intragastrically 40% ethanol (0.5 ml per 100 g body weight) in combination with 5 % and 10 % solution of ethyl alcohol, respectively, as drinking ad libitum for 9 weeks; group III – intact animals. Effect of chronic alcohol abuse on animal behavior was evaluated in the test «open field» every 10 days. Results and discussion. The toxic hepatitis development has been revealed on the basis of biochemical and histological analysis on the background of alcohol intoxication in rats. Degenerative changes of chondrocytes in the peripheral zone of cartilage were not found histologically, while the majority of chondrocytes in the area of mature cartilage were in the state of severe degradation. Therefore, it is concluded that alcoholic hepatitis with the destruction of mature cartilage of the femoral head develops in animals on the background of chronic (9 week) alcohol intoxication.
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About Authors (Correspondence):
Shusharin A.G. – candidate of medical sciences, head of the laboratory of regenerative medicine, assistant professor, e-mail: shurin54@yandex.ru
Biruykova M.S. – engineer of laboratory of pharmacological investigation, e-mail: Ya.marina.biryukova@mail.ru
Polovinka M.P. – candidate of chemical sciences, senior researcher of the laboratory of regenerative medicine, assistant professor, e-mail: marinapolovinka@yandex.ru
Zhukova N.A. – doctor of medical sciences, senior researcher of the laboratory of pharmacological investigation, e-mail: gna2004@ngs.ru
Tolstikova T.G. – doctor of biological sciences, head of the laboratory of pharmacological investigation, professor, e-mail: tg_tolstikova@mail.ru
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