Designing superproducer of recombinant human apolipoprotein A-I based on Escherichia coli cell
Author Affiliations1Research Institute of Biochemistry, 630117, Novosibirsk, Timakov str., 2.
2Research Institute of Biochemistry, 630117, Novosibirsk, Timakov str., 2.
3Research Institute of Biochemistry, 630117, Novosibirsk, Timakov str., 2.
Abstract
This article presents materials on the production of a chimeric recombinant polypeptide in E. coli cells and its transformation into a mature form of human apolipoprotein A-I. Glu2Asp mutation was introduced into the gene apolipoprotein A-I, it is allowed to cleave the leader sequence of the chimeric polypeptide by acid hydrolysis. Codons of recombinant DNA were optimized for expression in E. coli, resulting in the output of the chimeric polypeptide from the cell culture averaged 200 mg/l.
Key words
References
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About Authors (Correspondence):
Ryabchenko A.V. – candidate of biological sciences, senior researcher, laboratory of medical biotechnology, e-mail: borrelia@mail.ru
Kotova M.V. – postgraduate student, laboratory of medical biotechnology, e-mail: zerokiri@mail.ru
Polyakov L.M. – head of the laboratory of medical biotechnology, e-mail: plm@niibch.ru
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