The Siberian Scientific Medical Journal
№ 6 / 2015 / 16-21

Designing superproducer of recombinant human apolipoprotein A-I based on Escherichia coli cell

Author Affiliations


This article presents materials on the production of a chimeric recombinant polypeptide in E. coli cells and its transformation into a mature form of human apolipoprotein A-I. Glu2Asp mutation was introduced into the gene apolipoprotein A-I, it is allowed to cleave the leader sequence of the chimeric polypeptide by acid hydrolysis. Codons of recombinant DNA were optimized for expression in E. coli, resulting in the output of the chimeric polypeptide from the cell culture averaged 200 mg/l.

Key words

E. coli, apolipoprotein A-I, recombinant protein
About Authors (Correspondence):

Ryabchenko A.V. – candidate of biological sciences, senior researcher, laboratory of medical biotechnology, e-mail:

Kotova M.V. – postgraduate student, laboratory of medical biotechnology, e-mail:

Polyakov L.M. – head of the laboratory of medical biotechnology, e-mail:

Full Text

Received: 12/01/2016