Experimental aprobation of new biomedicine substence from pork kidneys at the experimental kidney stone disease
Abstract
The aim of present investigation was approbation in experimental kidney stone disease conditions antilitogenic action of new biomedicine substance from pork kidney. Experiments were performed on 30 male divided into 2 groups. In control group 1 % solution of ethylenglycole (EG) was given as drink for the rats during 6 weeks. The rats of therapy group were given EG during 6 weeks and the substance from raw material of pork kidneys was administrated in dose 1 g/rat from the 4-th week. Generating of substance from raw material of pork kidneys was carried out by the joint-stock company «Altayvitaminy». Technology of generating is based on lyophilic dehumidification. The oxalate, phosphate and calcium concentrations, activity of urothelium injury marker enzymes: lactate dehydrogenase (LDH), γ-glutamyl transferase (GGT), N-acetyl-β-D-glucose aminidase (NAG), and oxygen’s free radicals activity were determined every 3-4 days in both rats groups. The calcium deposits were evaluated in the rats kidney sections by Van Koss hystochemical method. Chronic use of EG in control group resulted in experimental kidney stone disease progression which was indicated by oxalate super saturation in urine, enhancing of marker enzymes activity, activation of oxidative stress in the kidney and in the blood, and forming calcium deposits near renal papilla. The treatment of experimental nephrolithiasis led to the great relief. It was indicated by the significant reduction of oxaluria, pronounced decrease of marker enzymes activity: LDH, GGT, NAG, and reduction of oxidative stress. Finally, it was confirmed by morphological studies, which detected very significant reduction of calcium deposits number and their sizes. The evidence of high antilitogenic activity of new biomedicine substance from pork kidney has been revealed as the result of the research.
Key words
ReferencesZharikov A.Yu. – doctor of biological sciences, professor of the chair for pharmacology, е-mail: zharikov_a_y@mail.ru
Lampatov V.V. – doctor of biological sciences, professor of the chair for pharmacology, e-mail: lampatov@asmu.ru
Bryukhanov V.M. – doctor of medical sciences, head of the chair for pharmacology, e-mail: bvm@agmu.ru
Talalaeva O.S. – candidate of medical sciences, assistant of professor of the chair for pharmacology, е-mail: talalaeva_olga@mail.ru
Motin Yu.G. – candidate of medical sciences, assistant of professor of the chair for pathological anatomy, e-mail: ymotin@mail.ru
Pavlyashik G.V. – e-mail: ganna1704@mail.ru
Eliseev D.V. – e-mail: dezoxyadenozin@gmail.ru
