The Siberian Scientific Medical Journal
 
 
№ 1 / 2016 / 66-69

Multiplex detection of IGG class antibodies to Toxoplasma gondii, Rubella virus, cytomegalovirus and Herpes simplex virus using a novel multiplex flow immunoassay

Author Affiliations

Abstract

Preclinical tests of the novel multiplex immunoassay for the detection of TORCH infections in human serum using flow cytometry are presented. The immunoassay is intended for the qualitative detection of specific human IgG class antibodies to Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus in human serum. Multiplex analysis is performed in vitro on polystyrene microspheres encoded with organic dye. The diagnostic sensitivity of the kit is evaluated to be not less than 95%.

Key words

TORCH complex, immunodiagnostics, immunofluorescence, bead-based multiplex immunoassay
References
About Authors (Correspondence):

Dolgushin S.A. – candidate of physical and mathematical sciences, senior researcher of the chair for biomedical systems, e-mail: dolgushin.sergey@gmail.com

Loseva E.M. – researcher, e-mail: losevaem@gmail.com

Odintsova E.S. – candidate of biological sciences, junior researcher, e-mail: odintsoval@ngs.ru

Tronin A.V. – director, e-mail: tronin@mbu-tech.com

Selishchev S.I. – doctor of physical and mathematical sciences, professor, headr of the chair for biomedical systems, e-mail: sersel@miee.ru 

Full Text

Received: 21/03/2016
Accepted: 01/01/1970