Influence of scopolamine, galantamine and their combination with hexametonium and atropine on the spectral characteristics of heart rhythm of nonlinear rats
Author Affiliations1Astrakhan State University, 414000, Аstrakhan, Schaumyan sq., 1
2Astrakhan State University, 414000, Аstrakhan, Schaumyan sq., 1
3Astrakhan State University, 414000, Аstrakhan, Schaumyan sq., 1
4Astrakhan State University, 414000, Аstrakhan, Schaumyan sq., 1
Abstract
We have studied wave characteristics of heart rate variability (HRV) of nonlinear male rats under the scopolamine (2 mg / kg b.w.), its combination with hexamethonium at the high dose (30 mg / kg b.w.), with galantamine (2 mg / kg b.w.), as well as at consistent blockade of N-cholinergic receptors of vegetative nodes (hexamethonium, 7 mg / kg b.w.) and M-cholinergic receptors (atropine, 1 mg / kg b.w.). Spectral analysis of HRV has been carried out in the following ranges: HF (0.9–3.5 Hz), LF (0.32–0.9 Hz), VLF (0.15–0.32 Hz). The injection of scopolamine decreases power, unlike galantamine which in some rate increases the power of the entire spectrum of HRV waves. Consequently, these drugs have not any specific influence of HRV waves. The blockade of N-cholinergic receptors of vegetative nodes sharply reduces the power of all HRV waves, especially LF and VLF. The most significant inhibition of HRV waves is triggered by the combination of scopolamine with hexamethonium (30 mg / kg). The injection of atropine supplies the effect of the cholinergic blockade and decreases the power of HRV waves of all frequency ranges, especially of HF. The atropine effects appear weaker after the preliminary injection of scopolamine and hexamethonium and are change on the background of galantamine. We suppose that the N-cholinergic transmission at the level of vegetative nodes responds for the spectral power of HRV, approximately at 75 % mainly at LF and VLF frequencies. M-cholinergic receptors localized in the heart, provide 15–20 % of the variability of RR-intervals, especially at HF-range. Therefore, cholinergic mechanisms of different levels are involved in the formation of the HRV wave’s power of all frequency ranges.
Key words
spectral analysis,
heart rate variability,
hexametonium,
high-frequency waves,
low-frequency waves,
very low-frequency waves,
blockade of M-cholinoreceptors,
blockade of N-cholinoreceptors,
blockade of acetylcholinesterase,
scopolamine,
galantamine References
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About Authors (Correspondence):
Kuryanova E.V. – doctor of bioogical sciences, professor of the chair for physiology, morphology, genetic and biomedicine, e-mail: fyzevk@rambler.ru
Zhukova Yu.D. – junior researcher of the research and education center at the chair for physiology, morphology, genetic and biomedicine, е-mail: iuliya.zhukova@mail.ru
Tryasuchev A.V. – postgraduate student of the chair for physiology, morphology, genetic and biomedicine, е-mail: tryndval@mail.ru
Horst N.A. – doctor of biological sciences, professor of the chair for physiology, morphology, genetic and biomedicine, e-mail: nagorst@mail.ru
