The Siberian Scientific Medical Journal
 
 
№ 4 / 2016 / 5-9

Structural analysis of biosynthesized cellulose based on the results of confocal microscopy

Author Affiliations

Abstract

Purpose. To study structural features of biosynthesized cellulose based on the results of the confocal microscopy. Material and design. Biosynthesized cellulose, purified from bacterial products, synthesized Gluconacetobacter xylinus S3, obtained as films with thickness of 1 to 2 mm was analyzed. Its native frozen sections were investigated using direct and inverted confocal microscopes LSM 710, LSM 780 NLO, produced by Carl Zeiss. Results and discussion. Fibers with diameter of 25–220 nm and length of 100 microns are structural base of synthesized cellulose. Macro-fibrious structures with diameter about 100 microns, formed by radially oriented microfibers are to be seen on cross sections.

Key words

bacterial cellulose, confocal microscopy, structural analysis
References
About Authors (Correspondence):

Larionov P.M. – doctor of medical sciences, professor, head of group of morphology and molecular genetic studies, e-mail:ptrl@mail.ru

Filippenko M.L. – candidate of biological sciences, head of laboratory of pharmacogenomics, e-mail: mlfilipenko@gmail.com

Stupak V.V. – doctor of medical sciences, professor, chief researcher of clinic of neurosurgery, e-mail: VStupak@niito.ru

Kharchenko A.V. – neurosurgeon of clinic of neurosurgery, e-mail: alexdok2000@rambler.ru

Afonyushkin V.N. – candidate of agricultural sciences, leading engineer of laboratory of pharmacogenomics

Tereshchenko V.P. – medical resident, e-mail: tervp@ngs.ru

Barinov A.A. – leading specialist in confocal microscopy, e-mail: Barinov@optecgroup.com

Received: 30/08/2016