Modern possibilities of cell technologies in biological equivalent of artificial cornea constructing

Purpose. To study the possibility of biological equivalent of artificial cornea constructing on the basis of biosynthetic protein spidroin with the use of contemporary 3D cell technologies. Material and methods. The cells of eye limbus after the second passage were planted on the agarose plate in the presence of the complete growth medium for gaining 3D spheroids. The solution of spidroin was used for constructing the matrices. Results and discussion. Both 3D spheroids and the microtissue had epithelial and mesenchymal components as well as regularly organized extracellular matrix. These facts altogether give the basis for construction of biological equivalent of artificial cornea.

Malyugin B.E. – doctor of medical sciences, professor, deputy of director for scientific studies

Borzenok S.A. – doctor of medical sciences, head of the center for basic and applied biomedical problems, professor of the chair for eye diseases, e-mail: mdborzenok@yandex.ru

Komakh Yu.A. – candidate of medical sciences, head of the laboratory of transplantation and cell biology

Arbukhanova P.M. – candidate of medical sciences, junior researcher of the laboratory of molecular physiology and pathology of vision

Zheltonozhko A.A. – ophthalmologist of Eye tissue bank, e-mail: salange@yandex.ru

Saburina I.N. – doctor of biological sciences, head of the laboratory of cell biology and pathology development

Kolokol’tsova T.D. – doctor of biological sciences, leading researcher of the laboratory of cell biology and pathology development, senior researcher of the laboratory of transplantation and cell biology

Zurina I.M. – junior researcher of the laboratory of cell biology and pathology development

Agapov I.I. – doctor of biological sciences, head of the laboratory of bionanotechnology

Bogush V.G. – candidate of biological sciences, leading researcher of the laboratory of protein engineering

Davydova L.I. – senior researcher of the laboratory of protein engineering