№ 4 / 2016 / 39-44

Study of apolipoprotein A-I as a carrier of plasmid DNA on tumor lymphoblasts model

Author: Ryabchenko A.V.¹, Kotova M.V.², Polyakov L.M.³

Abstract

The paper presents the study of the transfecting cells possibility in vitro tumor lymphoblasts of line U937 using apolipoprotein A-I (apoA-I). Cells were transfected with vectors expressing in animal cells the gene of green fluorescent protein (gfp) and gfp gene fused with a gene of human apoA-I. The paper shows the penetration of FITC-labeled apoA-I into tumor cells of lymphoblasts, however, the positive results was not revealed with any of the vectors used in the transfection.

Key words

apolipoprotein A-I, transfection, lymphoblasts, gfp

References

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About Authors (Correspondence):

Ryabchenko A.V. – candidate of biological sciences, senior researcher of laboratory of medical biotechnology, e-mail: borrelia@mail.ru

Kotova M.V. – postgraduate student of laboratory of medical biotechnology, e-mail: zerokiri@mail.ru

Polyakov L.M. – doctor of medical sciences, professor, head of the laboratory of medical biotechnology, e-mail: plm@niibch.ru

Received: 31/08/2016