Articular cartilage growth: changes in composition and functional pool of proteoglycans of knee joint cartilage of mini-pigs in an ontogenesis
Composition and links between proteoglycans/glycosaminoglycans (GAGs) and matrix components of knee cartilage in mini-pigs were studied using biomedical methods in dynamics of postnatal growth. Newborns and animals at the age of 14 days, 1, 2 and 6 months were used. Proteoglycans were extracted sequentially with 0,14 M NaCl, solution of 4M guanidine hydrochloride and papain. The amounts of sulfated GAGs, uronic acids, and galactose were determined. The accumulation of keratan sulfate, and the dynamical changes in the amount of proteoglycans and in the ratio of free molecules and those associated with collagen network were revealed in the tissue during the growth process. These changes reflect the expansive growth of tissue and strengthening of the collagen network with age.
About Authors (Correspondence):
Rusova T.V. – candidate of biological sciences, senior researcher of the laboratory experimental department, e-mail: TRusova@niito.ru
Voropaeva A.A. – candidate of biological sciences, researcher of laboratory experimental department, e-mail: Voropaeva2011@rambler.ru
Baitov V.S. – candidate of medical sciences, senior researcher of arthroplasty department, e-mail: VBaitov@1st.ru