INFLUENCE OF N-(4-METHYL-3-CHLOROPHENYL)-2-ACETOXY-3,5-DICHLOROBENZAMIDE ON THE ADULT AND JUVENILE WORMS OF O. FELINEUS UNDER IN VITRO AND IN VIVO CONDITIONS
Author Affiliations1Institute of Cytology and Genetics of SB RAS
2Institute of Cytology and Genetics of SB RAS
3Institute of Cytology and Genetics of SB RAS
4Institute of Cytology and Genetics of SB RAS; Novosibirsk State University
5Research Institute of Molecular Biology and Biophysics
6Institute of Cytology and Genetics of SB RAS
7Institute of Cytology and Genetics of SB RAS
8Saint Petersburg State Chemical Pharmaceutical Academy
9Institute of Cytology and Genetics of SB RAS;Research Institute of Molecular Biology and Biophysics
Abstract
The purpose was to determine the anthelmintic properties of the new substance from the class of salicylanilides – N-(4-methyl-3-chlorophenyl)-2-acetoxy-3,5-dichlorobenzamide (nadinate) on trematoda O. felineus under in vitro and in vivo techniques. In vivo studies, the effects of the substance on juvenile parasites in liver of inbred C57BL/6 mice and on adult worms in the liver of Syrian hamsters (Mesocricetus auratus) were evaluated. In vitro experiments, the sensitivity of adult and juvenile helminths was assessed by the IC50 and IC95 values of nadinate when inducing non-motility of worms. In experiments in vitro, the high sensitivity of adult helminths to this substance was established. Even more pronounced immobilizing effect of the nadinate was obtained in juvenile O. felineus individuals. In studies in vivo, the anthelmintic impact of nadinate on adult O. felineus worms has not been established. However, the juvenile parasites, like in vitro situation, were sensitive to the administration of this substance. An assumption is made about the possible protective anthelmintic properties of the compound tested.
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References
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About Authors (Correspondence):
Avgustinovich D.F. – doctor of biological sciences, associate professor, senior researcher of the laboratory of molecular mechanism of pathological processes, e-mail: avgust@bionet.nsc.ru
Full Text
Received: 28/06/2017
Accepted: 28/06/2017
