The Siberian Scientific Medical Journal
 
 
№ 6 / 2017 / 28-37

CALCIFICATION OF ELASTIN-CONTAINING XENOGENIC BIOMATERIALS: INFLUENCE OF CONSERVANTS AND BISPHOSPHONATES

Author Affiliations

Abstract

Objective: Histological evaluation of early stages of calcification of the walls of the porcine aorta and bovine jugular veins preserved with glutaraldehyde and diglycidyl ether of ethylene glycol and modified with amino-containing bisphosphonic compounds in the experimental model of subcutaneous implantation to rats. Materials and methods: subcutaneous implantation of biomaterial samples to rats for 10, 20 and 30 days with subsequent study of calcium deposits by light microscopy using Von Kossa staining. Results: Elastin and smooth muscle cells are the main substrates for the calcification of xeno-aortic and xenovenous walls. The accumulation of calcium in the aorta does not depend on the basic preservation. Treatment with a diepoxide inhibits the calcination of xenobium up to 30 days of the experiment. The anticalcium effect of immobilized bisphosphonates-pamidron and 2- (2¢-carboxyethyl-amino) ethylidene-1,1-bisphosphonic acids varies depending on the microstructure of the biomaterials, the basic preservation method and the chemical structure of the bisphosphonate.

Key words

anticalcium treatment, bisphosphonates, glutaraldehyde, diepoxide, cardiovascular xenoprosthesis, elastin-containing tissues
References
About Authors (Correspondence):

Timchenko T.P. – junior researcher of laboratory for orthotics of the center for new technologies, e-mail: t_timchenko@meshalkin.ru

Full Text

Received: 09/01/2018