CONGENITAL IMMUNITY VALUES IN PATIENTS WITH KNEE DEFORMING OSTEOARTHROSIS DEPENDING ON THE SIZE OF TIBIAL MEDIAL CONDYLE
The data of study on congenital immunity features in 70 patients with stage III of knee deforming osteoarthritis depending on the tibia medial condyle size have been presented. СD3–СD16+СD56+, СD14+HLA-DR were measured by the flow cytometry technique. Metabolic activity of neutrophils was evaluated by the reaction of nitroblue tetrazolium reduction using the light microscopy method. Leukocytes, monocytes, neutrophils were counted with a hematologic analyzer. The increase in stimulation HCT-test and serum IL-8 level were revealed in the group with osteoarthritis without condyle defect. The increase in metabolic potential of both resting and activated neutrophils and monocyte absolute number were registered in the group with minimal defect. HLA-DR expression on peripheral blood monocytes was additionally decreased and NK-cells content in synovial fluid was increased in the group with the defect of 50–70 % condyle area of 6–10-mm depth. The most marked changes in the studied values were revealed in the group with the defect of >70 % of single condyle of >10-mm depth. The obtained data testify to the important role of the immune-inflammatory component in osteoarthritis pathogenesis and they demonstrate the dependence of congenital immunity values on the size of medial condyle defect.
About Authors (Correspondence):
Kuznetsova E.I. – junior researcher of clinical laboratory of microbiology and immunology, e-mail: firstname.lastname@example.org