DOI 10.15372/SSMJ20180105
EFFECT OF SINGLE AND MULTIPLE IMPACT OF HYPOXIC HYPOXIA OF STRONG INTENSITY ON THE STATE OF THE HEMOSTATIC SYSTEM IN RATS
Author Affiliations1Altai State Medical University of Minzdrav of Russia 656038, Barnaul, Lenin av., 40
2Altai State Medical University of Minzdrav of Russia 656038, Barnaul, Lenin av., 40; Research Institute of Physiology and Fundamental Medicine 630117, Novosibirsk, Timakov str., 4
3Altai State Medical University of Minzdrav of Russia 656038, Barnaul, Lenin av., 40; Research Institute of Physiology and Fundamental Medicine 630117, Novosibirsk, Timakov str., 4
4Altai State Medical University of Minzdrav of Russia 656038, Barnaul, Lenin av., 40; Research Institute of Physiology and Fundamental Medicine 630117, Novosibirsk, Timakov str., 4
5Altai State Medical University of Minzdrav of Russia 656038, Barnaul, Lenin av., 40; Research Institute of Physiology and Fundamental Medicine 630117, Novosibirsk, Timakov str., 4
Abstract
The aim of the study was to analyze the state of the reaction of the hemostasis system in rats to a single and multiple exposures to hypoxic hypoxia of strong intensity. Material and methods. Male rats (40 individuals) of the Wistar line were used in the work. Animals have been subjected to a single / daily multiple hypoxic hypoxia by chamber ascent for 1 hour. Hypoxic hypoxia was modeled with the input-outlet chamber. The air discharge created in the pressure chamber in the training mode corresponded to the liftoff to the «height» of 7000 m above sea level (41.105 kPa, 308.3 mm Hg, hypoxia of strong intensity). Results and discussion. A single exposure to the strong intensity of hypoxic hypoxia is characterized by the state of thrombotic readiness (based on the revealed hypercoagulation and high level of markers of intravascular coagulation). The shortening of clot formation start time, increase in the α angle and the maximum bunch density were revealed according to the thromboelastography data. The activation of hemostasis system thrombocytic part, hypercoagulation through the internal clotting path with the thrombinemia signs disappearance have been revealed after completion of the 30-day cycle of severe intensity hypoxia. In addition, the increase in the blood plasma antithrombin reserve was found in the group of experimental animals. The lengthening of clot formation time was recorded by the thromboelastogram results.
Key words
ReferencesBarkagan Z.S., Momot A.P. Diagnosis and controlled therapy of hemostasis disorders. Moscow: N'yudiamed-AO, 2001. 306 p. [In Russian].
Chereshnev V.A., Yushkov B.G., Klimin V.G., Lebedeva E.V. Immunophysiology. Ekaterinburg, 2002. 260 p. [In Russian].
Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations of the member states regarding the protection of animals used for experimental and other purposes // Official Journal of the European Communities. 1986. L358. 1–29.
Goroshinskaja I.A., Nemashkalova L.A., Sorokina I.V. Effect of pyrazidol on the state of animals and monoamine content in periodic hypobaric hypoxia // Fiziologicheskiy zhurnal imeni Ivana Mikhaylovicha Sechenova = Journal of Physiology. 1993. (10). 67–73. [In Russian].
Makarenko A.N., Karandeeva Yu.K. Adaptation to hypoxia as a protective mechanism in pathological conditions // Visnik problem biologii i meditsini = Bulletin of Problems in Biology and Medicine. 2013. (2). 27–33. [In Russian].
Mamadalieva N.I., Saatov T.S., Hajbullina Z.R., Umerov O.I. The influence of pharmacorrection on the activity of protective enzymes against reactive oxygen species in the heart when adapting to hypoxia of varying intensity and duration // Vestnik Novosibirskogo gosudarstvennogo pedagogicheskogo universiteta = The Novosibirsk State Pedagogical University Вulletin. 2014. (1). 221-229. [In Russian].
Shahmatov I.I., Kiselev V.I., Vdovin V.M. The state of hemostasis system after application of different types of hypoxia // Byulleten' Sibirskogo otdeleniya Rossiyskoy akademii meditsinskikh nauk = Bulletin of Siberian Branch of Russian Academy of Medical Sciences. 2010. 30. (2). 131-137. [In Russian].
Shakhmatov I.I., Vdovin V.M., Bondarchuk Yu.A., Alekseeva O.V., Kiselev V.I. Hypoxic hypoxia as a factor that activates the hemostasis system // Byulleten' sibirskoy meditsiny = Bulletin of Siberian Medicine. 2007. № 1. 67-73. [In Russian].
About Authors (Correspondence):
Moskalenko S.V. – lecturer of the chair for normal physiology, e-mail: sunrisemsv@gmail.com
Full Text
Received: 26/02/2018
Accepted: 26/02/2018
