The results of microbiological study on the inflammation purulent focus in patients with chronic hematogenous osteomyelitis

Microbiological investigation of 24 patients with diagnosed long bone chronic hematogenous osteomyelitis in the exacerbation phase was carried out. 24 samples were taken, 30 strains of different bacteria were isolated. Quantitative microbiological investigations revealed high microbial dissemination of wounds in 75 % of patients. Microflora was isolated in monoculture in most cases - 79.2 %. Staphylococci domination (70.0 %) was a distinctive feature, and what is more, Staphylococcus aureus accounted for 63.3 %. Staphylococci were high sensitive to Rifampicin, Gentamicin, Oxacillin, Ciprofloxacin, and 71.4-90.9 % of the strains revealed were sensitive to these preparations. The density of Methicillin-resistant S. aureus and S. epidermidis among staphylococci has been established to be 19.0 and 9.5 %, respectively.

1.    Abaev Yu. K., Adarchenko A. A., Zafranskaya M. M. Purulent surgery in childhood: changing perspectives // Detskaya khirurgiya. 2004. (6). 4-7. (in Russian)
2.    Bordiyan S. G., Malyshev M. G., Shiryaev N. D. The features of hematogenous osteomyelitis in children of the Arkhangelsk region // Ekologiya cheloveka. 2006. (6). 37-40. (in Russian)
3.    Esina O.V., Shtofin S.G. The results of surgical treatment of patients with chronic osteomyelitis using titanium nickelide // Sibirskoe meditsinskoe obozrenie. 2010. 65. (5). 63-66. (in Russian)
4.    Ishutov I.V., Alekseev D.G. The main principles of ozonotherapy in treatment of patients with chronic osteomyelitis // Vestnik eksperimental'no? i klinichesko? khirurgii. 2011. IV. (2). 314-320. (in Russian)
5.    V.M.Dobrynin, I.A.Dobrynina, V.V.Katsalukha et al. Technical manual on microbiological diagnosis of wound infections in the treatment-and-diagnostic institutions of the Army and Navy // SPb. NII epidemiologii i mikrobiologii im. Pastera. 1999. 4-62. (in Russian)
6.    On the unification of microbiological (bacteriological) methods of study used in clinical-and-diagnostic laboratories of treatment-and-prophylactic institutions: Prikaz N 535 MZ SSSR. M., 1985. 126 s. (in Russian)
7.    Determination of microorganism sensitivity to antibacterial preparations: Guidelines of MUK 4. 2. 1890 - 04 // Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya. 2004. 6. (4). 306-359. (in Russian)
8.    Okulich V.K., Fedyanin S.D., Plotnikov F.V., Shilin V.E., Matskevich E.L. Efficient use of antibitics in treatment of hematogenous and posttraumatic osteomyelitis // Novosti khirurgii. 2009. 17. (4). 65-67. (in Russian)
9.    Sonis A.G. New in diagnosis and treatment of osteomyelitis: abstract of thesis … doctor of medical sciences. Samara, 2010. (in Russian)
10.    Khasanov A.G., Nurtdinov M.A., Nigmatzyanov S.S., Sakaev R.Sh. The results of using plasma flows in complex treatment of chronic osteomyelitis // Vestnik eksperimental'no? i klinichesko? khirurgii. 2010. III. (3). 207-209. (in Russian)
11.    Shchitinin V. E., Arapova A. V., Korovin S. A. Acute hematogenous osteomyelitis in children. M., 2000. 40 p. (in Russian)
12.    Bonhoeffer J., Haeberle B., Schaad U.B., Heininger U. Diagnosis of acute haematogenous
osteomyelitis and septic arthritis: 20 years experience at the University Children's Hospital

Rozova L.V. - junior researcher of scientific clinical laboratory of microbiology and immunology
Naumenko Z.S. - candidate of biological sciences, head of scientific clinical laboratory of microbiology and immunology
Godovykh N.V. - junior researcher of scientific clinical laboratory of microbiology and immunology, e-mail: natalia_nvn@mail.ru