Cyclophosphamide administration for limitation of carcinosarcoma Walker 256 growth in rats aggravates the paraneoplastic ultractructural disturbances in the liver
Author Affiliations1Novosibirsk State Medical University 630091, Novosibirsk, Krasny av., 52
2Novosibirsk State Medical University of Federal Agency of Public Health and Social Development 630009, Novosibirsk, Krasnyi av., 52
3Novosibirsk State Medical University of Minzdrav of Russia 630091, Novosibirsk, Krasny av., 52
4Novosibirsk State Medical University 630091, Novosibirsk, Krasny av., 52
5Novosibirsk State Medical University of Minzdrav of Russia 630091, Novosibirsk, Krasny av., 52
Abstract
The signs of lysis and necrobiosis intensification in parenchyma and stroma cells were revealed in the liver of carcinosarcoma Walker 256-bearing rats at the 7th day after cyclophosphamide administration. Compensatory and adaptive reconstructions and apoptosis were noted in part of cell as a result of cyclophosphamide cytostatic effect. Combined effects of paraneoplastic factors and cyclophosphamide enhance subcellular sinusoidal obstructive syndrome manifestation caused by cyclophosphamide. Hepatocytes ultrastructural features reflect the functional liver response via triggering of detoxification mechanisms in hepatocytes with extensive smooth endoplasmatic reticulum.
Key words
References1. Gantsev Sh.Kh. Oncology. Moscow, 2004. 516 p. (in Russian)
2. Oncology: national instruction. Moscow, 2008. 1072 p. (in Russian)
3. Wang X., Kanel G.C., DeLeve L.D. Support of sinusoidal endothelial cell glutathione prevents hepatic veno-occlusive disease in the rat // Hepatology. 2000. 31. 428–434.
4. Sulkowska M., Sulkowski S., Skrzydlewska E. The effect of pentoxifylline on ultrastructure and antioxidant potential during cyclophosphamide-induced liver injury // J. Submic. Сytol. Pathol. 1999. 31. (3). 413–422.
5. Khegay I. I., Popova N. A., Ivanova L. N. Vasopressin expression influence on WALKER-256 carcinosarcoma growth // Genetika. 2000. 42. (7). 993–995.
6. Ding Z., Lambrechts A., Parepally M., Roy P. Silencing profilin-1 inhibits endothelial cell proliferation, migration and cord morphogenesis // J. Cell Sci. 2006. 119. (19). 4127–4137.
7. Dong Z., Saikumar P., Patel Y., Weinberg J. M., Venkatachalam M.A. Serine protease inhibitors suppress cytochrome c-mediated caspase-9 activation and apoptosis during hypoxia–reoxygenation // Biochem. J. 2000. 347. (3).669–677.
8. Ruoslahti E., Reed J.C. Anchorage dependence, integrins, and apoptosis // Cell. 1994. 77. (4). 477–478.
9. Gressner A.M. Transdifferentiation of hepatic stellate (Ito) cells to myofibroblasts: a key event in hepatic fibrogenesis // Kidney Int. 1996. 49. (1). 39–45.
10. McAuslan B.R., Hoffman H. Endothelium stimulating factor from Walker carcinoma cells: Relation to tumor angiogenic factor // Exp. Cell Res. 1979. 119. (1). 181–190.
About Authors (Correspondence):
Efremov A.V. ─ doctor of medical sciences, professor, Corresponding Member of RAMN, head of the chair of pathophysiology, honored scientist of the RF
Kunts T.A. ─ researcher of Central Research Laboratory, e-mail: tkunts@ngs.ru
Ovsyanko E.V. ─ candidate of medical sciences, associated professor of the chair of anatomy, histology, biology
Ishenko I.Yu. ─ candidate of biological sciences, researcher of Central Research Laboratory
Pustovetova M.G. ─ doctor of medical sciences, professor, head of Central Research Laboratory
Full Text
