Isolation and analysis of high-polymer rna biological activity from baker’s yeast
Author Affiliations1LLC «Vitalang»
2Novosibirsk State Pedagogical University
3Scientific Research Institute of Biochemistry SB RAMS
Abstract
A new method for an amphiphilic high-polymer RNA producing from dry baker’s yeast is elaborated. The main effect of cheapening and non-waste production is achieved by use of oleic acid (food detergent) as lytic agent at the concentration of 1–2 %. In comparison with known analogues, characterized by the use of 2-ethylhexanoic acid as highly lytic agent, the proposed method does not require a deep cleaning of the final product. The introduction of dry yeast in a cool boiling solution of the lytic agent, without their preliminary soaking can suppress the activity of lysosomal ribonucleases. Non-waste production is that together with the high-polymer RNA, a concentrate feed protein, low molecular weight RNA and natural oligoribonucleotides can be obtained. Experimental verification of the antiviral activity of amphiphilic high-polymer RNA is performed on outbred mice infected with ectromelia virus. It is shown that intramuscular administration of therapeutic doses of preparation to mice causes marked protection from 10 semi-lethal dose (LD50) of ectromelia virus that ranges from 28.6 to 47.6 % depending on the dose. Introduction of preventive doses of preparation leads to greater protection of mice against infection. The protective effect of the preparation has dose-dependent character: the most significant effect is observed at the dose of 1,0 mg/kg of body weight: in this case 61.9 % of animals are survives in experiment, 19.1 % – in control group.
Key words
References
- Patent 2045270 RF. Specific stimulator of protein biosynthesis in hematopoietic and immunocompetent bodies / Panin L.E., Khar'kovskiy A.V.; published 10.10.95. (in Russian)
- Sokolov V.D., Andreeva N.L., Sokolov A.V. Immunostimulators in veterinary medicine / / Veterinariya. 1992. (7–8). 49–50. (in Russian)
- Zemskov V.M., Lidak M.Yu., Zemskov A.M., Mikstays U.Ya. Low molecular weight RNA: preparation, hydrolysis and medical applications. Riga: Zinatne, 1985. 191 p. (in Russian)
- Yamkovaya T.V., Yamkovoy V.I., Panin L.E. The biological activity of different preparations of RNA from baker's yeast // Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk 2006. (3). 117–121. (in Russian)
- Knorroz M.Yu., Popova O.M., Davydova A.A. et al. A comparative study of antiviral activity of natural double-stranded RNA of tick-borne encephalitis in an experimental // Voprosy virusologii. 1985. 30. (6). 697–700. (in Russian)
- RNA towards medicine. Series: handbook of experimental pharmacology. Vol. 173. Eds. V.A. Erdmann, J. Brosius, J. Barciszewski. N.Y.: Springer-Verlag, 2006. XII. 464 p.
- Redis R.S., Berindan-Neagoe I., Pop V.I., Calin G.A. Non-coding RNAs as theranostics in human cancers // J . Cell. Biochem. 2012. 113. (5). 1451–1459.
- Krylov I.A., Markina N.S., Krasnoshtanova A.A. et al. Selection of optimal schemes of obtaining yeast RNA in modern biochemical factories BVK and lysine // Biotechnologiya. 1992. (2). 81–83. (in Russian)
- Davis F.F., Allen F.W. Ribonucleic acids from yeast which contain a fifth nucleotide // J. Biol. Chem. 1957. 227. (2). 907–915.
- Yamkovaya T.V., Homov V.V., Zagrebel'nyy S.N., Yamkovoy V.I. Isolation of total RNA from baker's yeast // Prikladnaya biokhimiya i mikrobiologiya. 2006. 42. (1). 93–97. (in Russian)
- European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes. Strasbourg, 1986. (in Russian)
- Crestfield A.M., Smith K.S., Allen F.W. The preparation and characterization of ribonucleic acids from yeast // J. Biol. Chem. 1955. 216. (1). 185–193.
- Patent 2436583 RF. Antiviral agent / Yamkovaya T.V., Zagrebel'nyy S.N., Panin L.E., Yamkovoy V.I.; published 20.12.2011. (in Russian)
- Patent 2392329 RF. Method of producing high-polymeric RNA from yeast / Yamkovaya T.V., Kuzovkova E.V., Zheleznova Yu.P. et al.; published 06.20.2010. (in Russian)
- Patent 2403288 RF. Method of producing high-polymeric RNA from dry baker's yeast / Yamkovaya T.V., Zagrebel'nyy S.N., Panin L.E., Yamkovoy V.I.; published 10.11.2010. (in Russian)
About Authors (Correspondence):
Yamkovaya T.V. – candidate of biological sciences, e-mail: yam_tv@mail.ru
Yamkovoy V.I. – doctor of biological sciences , professor of the chair for chemistry, e-mail: vitalang2@mail.ru
Panin L.E. – doctor of medical sciences, professor, academician of RAMS, director
Full Text