The Siberian Scientific Medical Journal
 
 
№ 6 / 2012 / 60-68

Isolation and analysis of high-polymer rna biological activity from baker’s yeast

Author Affiliations

Abstract

A new method for an amphiphilic high-polymer RNA producing from dry baker’s yeast is elaborated. The main effect of cheapening and non-waste production is achieved by use of oleic acid (food detergent) as lytic agent at the concentration of 1–2 %. In comparison with known analogues, characterized by the use of 2-ethylhexanoic acid as highly lytic agent, the proposed method does not require a deep cleaning of the final product. The introduction of dry yeast in a cool boiling solution of the lytic agent, without their preliminary soaking can suppress the activity of lysosomal ribonucleases. Non-waste production is that together with the high-polymer RNA, a concentrate feed protein, low molecular weight RNA and natural oligoribonucleotides can be obtained. Experimental verification of the antiviral activity of amphiphilic high-polymer RNA is performed on outbred mice infected with ectromelia virus. It is shown that intramuscular administration of therapeutic doses of preparation to mice causes marked protection from 10 semi-lethal dose (LD50) of ectromelia virus that ranges from 28.6 to 47.6 % depending on the dose. Introduction of preventive doses of preparation leads to greater protection of mice against infection. The protective effect of the preparation has dose-dependent character: the most significant effect is observed at the dose of 1,0 mg/kg of body weight: in this case 61.9 % of animals are survives in experiment, 19.1 % – in control group.

Key words

ectromelia virus, high-polymeric RNA, yeast, oleic acid, antiviral properties.
References
About Authors (Correspondence):

Yamkovaya T.V. – candidate of biological sciences, e-mail: yam_tv@mail.ru

Yamkovoy V.I. – doctor of biological sciences , professor of the chair for chemistry, e-mail: vitalang2@mail.ru

Panin L.E. – doctor of medical sciences, professor, academician of RAMS, director

Full Text

Received: 09/02/2015