Study of the effects of recombinant TNF-binding variola virus protein in model of contact dermatitis
Author Affiliations1Institute of Clinical Immunology of SB RAMS 630099, Novosibirsk, Yadrintsevskaya str., 14
2Institute of Clinical Immunology of SB RAMS 630099, Novosibirsk, Yadrintsevskaya str., 14
3Scientific Center of Virology and Biotechnology "Vector" of Rospotrebnadzor 630559, Novosibirsk region, Koltsovo
4Institute of Clinical Immunology of SB RAMS 630099, Novosibirsk, Yadrintsevskaya str., 14,Novosibirsk State Medical University of Minzdrav of Russia 630091, Novosibirsk, Krasny av., 52
5Scientific Center of Virology and Biotechnology "Vector" of Rospotrebnadzor 630559, Novosibirsk region, Koltsovo
6Institute of Clinical Immunology of SB RAMS 630099, Novosibirsk, Yadrintsevskaya str., 14
Abstract
The effects of applications of recombinant TNF-binding variola virus protein VARV-CrmB in model of contact dermatitis have been investigated. It is shown that VARV-CrmB significantly reduces dinitrochlorbenzene (DNCB)-induced migration of leukocytes (dendritic cells/Langerhans cells) in the afferent phase of contact reaction and the indicator "value ear edema" in the efferent phase of contact reaction. It is known that during inflammation population of proliferating and migrating Langerhans cells replenished by recruitment of bone marrow precursors. Study of bone marrow hematopoiesis in contact dermatitis model showed that the coapplication of DNCB+VARV-CrmB significantly reduced DNCB-induced increase in the number of granulocyte-macrophage colony up to control values. The obtained results suggest the possibility of use of TNF blockers by application in the treatment of inflammatory diseases of the skin.
Key words
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About Authors (Correspondence):
Viazovaia E.A. - candidate of biological sciences, senior researcher of laboratory of stem cell immunobiology, e-mail: viazovaia@mail.ru
Toporkova L.B. - candidate of biological sciences, senior researcher of laboratory f stem cell immunobiology, e-mail: toporkova12@mail.ru
Gileva I.P. - doctor of biological sciences, leading researcher of department of genomic research and development of methods of DNA diagnosis of poksviruses, е-mail: gileva@vector.nsc.ru
Tsyrendorzhieva M.D. - laboratory assistant researcher of laboratory of stem cell immunobiology and student of medical faculty, e-mail: mashats_lovely@mail.ru
Shchelkunov S.N. - doctor of biological science, head of department of genomic research and development of methods of DNA diagnosis of poksviruses, е-mail: snshchel@vector.nsc.ru
Orlovskaya I.A. - doctor of medical sciences, professor, head of laboratory of stem cell immunobiology, e-mail: irorl@mail.ru
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