№ 6 / 2012 / 77-83

Species of Borrelia Burgdorferi Sensu Lato spirochetes, circu-lating in Ixodes Persulcatus ticks population at the territory of Novosibirsk scientific center

Author: Beklemishev A.B.¹, Ryabchenko A.V.², Mamaev A.L³

Abstract

The aim of the study – to determine the infection rate by spirochetes B. burgdorferi sl of I. persulcatus ticks, collected in the recreational zone of Novosibirsk in the spring periods from 2003 to 2008. Material and methods. Detection and genotyping borrelia in ticks was performed by PCR and by analysis of restriction fragments length polymorphism of amplicons from rrf-rrl intergenic region. Results. A total of 1259 individuals of adult ticks were analyzed. The infection rate of ticks by borrelia in the period from 2003 to 2008 ranged from 20.1 ± 2.3 to 31.7 ± 6.0 %. Two species of spirochetes were found in ticks: B. afzelii and B. garinii. The proportion of B. afzelii spirochetes was relatively constant, whereas in the case of B. garinii species, consisting of NT29 and 20047^T genomic groups, observed increase in the proportion of Borrelia 20047^T group. It was identified individual ticks infected presumably with B. japonica spirochetes.

Key words

Borrelia burgdorferi sensu lato., PCR,, species, infection rate, Ixodes persulcatus

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About Authors (Correspondence):

Beklemishev A.B. – doctor of biological sciences, professor, head of the laboratory, e-mail: beklem@soramn.ru

Ryabchenko A.V. – candidate of biological sciences, senior researcher, e-mail: masterbio@soramn.ru

Mamaev A.L. – researcher, e-mail: alexeymamaev@inbox.ru

Full Text

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Received: 10/02/2015