The variants of neoplastic proliferation in initially phenotypic different experimental lymphosarcomas
Author Affiliations1Novosibirsk State Medical University of Minzdrav of Russia 630091, Novosibirsk, Krasnyi av., 52
2Institute of Chemical Biology and Fundamental Medicine SB RAS 630090, Novosibirsk, Akademic Lavrent’yev av., 8
3Novosibirsk State Medical University of Minzdrav of Russia 630091, Novosibirsk, Krasnyi av., 52
4Institute of Chemical Biology and Fundamental Medicine SB RAS 630090, Novosibirsk, Akademic Lavrent’yev av., 8
Abstract
The effects of polychemotherapy (PCT) on the tumor tissue in transplanted tumors RLS and RLS40 manifested a phenotype of resistance to cytotoxic therapy have been investigated in the study. After the 3-course of polychemotherapy exposure to tumors RLS the level of gene expression increased: for mdr1b – in 1.8 times, for bcl-2 – in 1.3 times; after the exposure to tumor RLS40 the gene expression indices increased more significantly. The volume density of apoptosis in RLS40 remained unchanged after polychemotherapy but in RLS the number of apoptosis increased from course to course. The 4th courses of polychemotherapy realization was accompanied with the increase in expression of proliferation marker Ki-67 in tumor cells RLS, that testified to the intensification of proliferative regime in tumor which supported the neoplasm increases against the background of apoptosis active processes in it.
Key words
References1. Pospelova T.I., Loseva M.I., Kovynev I.B. et al. Basis of tumor progression of hemoblastosis // Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk. 2004. (2).73–76. (in Russian)
2. Fuller G.M., Shields D. Molecular basis of medical cell biology. Stamford: Appleton & Lange, 1998. 231 p.
3. Filipits M. Mechanisms of cancer: multidrug resistance // Drug Discov. Today. 2004. 1. 229–234.
4. Stavrovskaya A.A. Cellular mechanisms of multidrug resistance of tumor cells // Biokhimiya. 2000. 65. (1). 112–126. (in Russian)
5. Sen'kova A.V., Ageeva T.A., Zenkova M.A. Mutual relation of processes of progressing of plural medicinal resistance in the tumour and morphological changes in the liver at mice with intertwined lymphosarcoma RLS 40 in the conditions of polychemotherapy // Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk. 2011. (2). 87–93. (in Russian)
6. Mironova N., Shklyaeva O., Andreeva E. et al. Animal model of drug-resistant tumor progression // Ann. N.Y. Acad. Sci. 2006. 1091. 490–500.
7. Sarckisov D.S., Perov Yu.L. Microscopic technique. Moscow, 1996. 542 p. (in Russian)
8. Avtandilov G.G. Medical morphometry. Moscow, 1990. 382 p. (in Russian)
9. Weibel E.R. Stereological methods. London: Acad. Press., 1979. 415 p.
10. Dabbs D.J. Diagnostic immunohistochemistry. Edinburgh: Churchill Livingstone, 2006. 848 p.
About Authors (Correspondence):
Voronina E.I. – postgraduate student of the chair for pathological anatomy, e-mail: e.voronina83@mail.ru
Sen,kova A.V. – candidate of medical sciences, researcher of the laboratory for nucleonic acids,
e-mail: alsenko@mail.ru
Ageeva T.A. – doctor of medical sciences, professor of the chair for pathological anatomy, e-mail: ageta@mail.ru
Zenkova M.A. – doctor of biological sciences, professor, head of the laboratory for nucleonic acids,
e-mail: marzen@niboch.nsc.ru
Full Text
