Author Affiliations1The S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, 127486, Moscow, Beskudnikovsky Blvd, 59a
2The S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, 127486, Moscow, Beskudnikovsky Blvd, 59a
3The S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, 127486, Moscow, Beskudnikovsky Blvd, 59a
4The Research Institute of General Pathology and Pathophysiology of RAMS, 125315, Moscow, Baltiyskaya str., 8
5The Research Institute of General Pathology and Pathophysiology of RAMS, 125315, Moscow, Baltiyskaya str., 8
6The Research Institute of General Pathology and Pathophysiology of RAMS, 125315, Moscow, Baltiyskaya str., 8
1. Achilli T.M., Meyer J., Morgan J.R. Advances in the formation, use and understanding of multi-cellular spheroids // Expert Opin. Biol. Ther. 2012. (10). 1347–1360.
2. Caramoy A. A reply to human retinal pigment epithelium (RPE) transplantation: outcome after autologous RPE-choroid sheet and RPE cell-suspension in a randomised clinical study // Br. J. Ophthalmol. 2011. 95. (3). 431.
3. Cheng N.C., Chen S.Y., Li J.R., Young T.H. Short-term spheroid formation enhances the regenerative capacity of adipose-derived stem cells by promoting stemness, angiogenesis, and chemotaxis // Stem Cells Transl. Med. 2013. 2. (8). 584–594.
4. Diniz B., Thomas P., Thomas B. et al. Subretinal implantation of retinal pigment epithelial cells derived from human embryonic stem cells: improved survival when implanted as a monolayer // Invest. Ophthalmol. Vis. Sci. 2013. 54. (7). 5087–5096.
5. Falkner-Radler C.I., Krebs I., Glittenberg C. et al. Human retinal pigment epithelium (RPE) transplantation: outcome after autologous RPE-choroid sheet and RPE cell-suspension in a randomised clinical study // Br. J. Ophthalmol. 2011. 95. (3). 370–375.
6. Foty R. A simple hanging drop cell culture protocol for generation of 3D spheroids // J. Vis. Exp. 2011. 6. (51). pii. 2720.
7. Lin R.Z., Chang H.Y. Recent advances in three-dimensional multicellular spheroid culture for biomedical research // Biotechnol. J. 2008. (9–10). 1172–1184.
8. Tezel T.H., Del Priore L.V. Reattachment to a substrate prevents apoptosis of human retinal pigment epithelium // Graefes Arch. Clin. Exp. Ophthalmol. 1997. 235. (1). 41–47.
9. Thomson H.A., Treharne A.J., Backholer L.S. et al. Biodegradable poly(α-hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation // J. Biomed. Mater. Res. A. 2010. 15. 95. (4). 1233–1243.
10. Uebersax E.D., Grindstaff R.D., Defoe D.M. Survival of the retinal pigment epithelium in vitro: comparison of freshly isolated and subcultured cells // Exp. Eye Res. 2000. 70. (3). 381–390.
11. Yaji N., Yamato M., Yang J. et al. Transplantation of tissue-engineered retinal pigment epithelial cell sheets in a rabbit model // Biomaterials. 2009. 30. (5). 797–803.
About Authors (Correspondence):
Borzenok S.A. – doctor of medical sciences, head of the center of fundamental and applicative medical biological problems, professor of the chair of eye diseases, e-mail: mdborzenok@narod.ru.
Popov I.A. – postgraduate student of the center of fundamental and applicative medical biological problems, e-mail: ilya_doctor_popov@mail.ru.
Ostrovskiy D.S. – junior researcher of the center of fundamental and applicative medical biological problems
Saburina I.N. – doctor of biological sciences, head of the laboratory of cell biology and developmental pathology, professor of the chair of pathophysiology
Kosheleva A.V. – candidate of biological sciences, leading researcher of the laboratory of cell biology and developmental pathology
Zurina I.M. – junior researcher of the laboratory of cell biology and developmental pathology
