The Siberian Scientific Medical Journal
 
 
№ 3 / 2014 / 42-47

The construction of transplants of human cadaveric donor retinal pigment epithelium by the method of 3D cellular culture

Author Affiliations

Abstract

AIM. To investigate the process of donor retinal pigment epithelium transplants as the spheroids (round cell masses). MATERIALS AND METHODS. The cells of donor retinal pigment epithelium were successively cultivated in two-dimensional and then in three-dimensional conditions. Methods of light microscopy, digital photography and image processing were used. RESULTS AND DISCUSSION. During the first 48 hours of three-dimensional cultivation spheroids of different morphology were forming. Spheroids with «smooth» surface showed greater fluidity in comparison to the spheroids with «rough» surface.

Key words

hanging drop, two-dimensional culture, retinal pigment epithelium, spheroid, transplant, three-dimensional culture
References
About Authors (Correspondence):

Borzenok S.A. – doctor of medical sciences, head of the center of fundamental and applicative medical biological problems, professor of the chair of eye diseases, e-mail: mdborzenok@narod.ru.

Popov I.A. – postgraduate student of the center of fundamental and applicative medical biological problems, e-mail: ilya_doctor_popov@mail.ru.

Ostrovskiy D.S. – junior researcher of the center of fundamental and applicative medical biological problems

Saburina I.N. – doctor of biological sciences, head of the laboratory of cell biology and developmental pathology, professor of the chair of pathophysiology

Kosheleva A.V. – candidate of biological sciences, leading researcher of the laboratory of cell biology and developmental pathology

Zurina I.M. – junior researcher of the laboratory of cell biology and developmental pathology

Full Text

Received: 09/02/2015