The Siberian Scientific Medical Journal
 
 
№ 4 / 2013 / 65-70

Genetic markers of severity of coronary vessels stenosis in patients with acute coronary syndrome

Author Affiliations

Abstract

To investigate the association of SNP (identified in recent GWAS) with single and multi-vessel coronary arteries damage in patients with acute coronary syndrome (ACS). Two hundred eighty patients with ACS have been admitted to the intensive care unit at City Clinical Hospital № 1 (Novosibirsk). 175 men (mean age 56.2 ± 5.2 years), and 105 women (mean age – 62.1 ± 5.3 years) were examined. The diagnosis of ACS was defined according to the set of criteria developed by the European Society of Cardiology and American College of Cardiology (2000). Ventriculo–coronarography was performed in 1–3 days after ACS symptoms onset. Genomic DNA was extracted from venous blood by phenol-chloroform extraction. Polymorphism of genes tested by real-time PCR according to the protocol producer firms (probes TaqMan, Applied Biosystems, USA). The following SNPs: rs499818, rs619203, rs10757278, rs1333049, rs1376251, rs2549513, rs4804611, rs17465637 were taken for investigation. The association was found between rs10757278 and, rs1333049 and multivessel coronary artery disease in patients with ACS both in the total group and in case of gender differentiation. For rs499818 – only in the total group. The association of rs2549513 with degree of coronary artery lesions was revealed only for men. For the first time the association of SNPs rs10757278, rs499818, rs2549513, rs1333049 with the degree of coronary artery disease was detected in Russian population. In the future, these genetic markers can be used as a part of risk measuring research to assess the risk of complications in patients with ACS in Russian population.

Key words

rs10757278, rs1333049, rs1376251, rs17465637, rs2549513, rs4804611, rs499818, rs619203, acute coronary syndrome (ACS), coronary arteries damage
References
About Authors (Correspondence):

Kulikov I.V. – candidate of medical sciences, senior researcher of the laboratory of molecular genetic studies of therapeutic diseases, e-mail: 248945@mail.ru

Lozhkina N.G. – candidate of medical sciences, assistant professor of the chair of internal diseases, e-mail: lozhkina.n@mail.ru

Maksimov V.N. – doctor of medical sciences, head of laboratory of molecular genetic studies of therapeutic diseases, e-mail: medik11@mail.ru

Orlov P.S. – junior researcher of the laboratory of molecular basis of animal genetics, e-mail: orlovpavel86@gmail.com

Kuimov A.D. – doctor of medical sciences, professor, head of the chair of internal diseases, e-mail: terapia@mail.ru

Voevoda M.I. – doctor of medical sciences, professor, corresponding member of RAMS, director, e-mail: mvoevoda@ya.ru

Full Text

Received: 02/02/2015