Bifidobacterium species range and microbial landscape of the large intestine in children with functional disorders of the digestive system
Author Affiliations1Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
2Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
3Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
4Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
5Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
6Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
7Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
8Scientific Center of Family Health and Human Reproduction Problems of SB RAMS, 664025, Irkutsk, Timiryazev str., 16
Abstract
The main objective of this work was to study bifidobacteria species range by means of method of polymerase chain reaction in different age children with functional gastro-intestinal tract disorders. General intestine microecological characteristics in children were studied too. Disbiotic changes in the intestinal biotope were accompanied by bifidobacteria deficiency and opportunistic microorganisms selection. Reduction of B.longum and B.catenulatum populations, the appearance of bifidobacteria rare species B. dentium и B. angulatum were shown. Decrease in bifidobacteria species diversity in children with functional gastro-intestinal tract disorders indicated instability in gut microecosystem. The study of bifidobacterial profiles in different pathologies will contribute to the early diagnosis and diseases treatment.
Key words
References
- Zhilenkova O.G. Breeding production of promising strains of bifidobacteria isolated from children. Dis ... kand. biol. Science. Moskva.2011. (in Russian)
- Zaprudnov A.M., Grigor'ev K.I. Modern features teenage gastroenterology. Pediatrics.2011;90(2):6-13. (in Russian)
- Kulagina E.V., Shkoporov A.N., Kafarskaja L.I. et al. The study of species and diversity of strains of bifidobacteria in the intestinal microflora populations of mothers and infants by using molecular genetic techniques// Bjulleten' jeksperimental'noj biologii i mediciny.2010;150:7:70-73/ (in Russian)
- Levanova L. A. Intestinal microenvironment residents of western Siberia, the correction dysbiotic states: Dis .... Dr. med. Science. Moskva.2003. (in Russian)
- Onishhenko G.G., Aleshkin V.A., Afanas'ev S.S., Pospelova V.V. Levanova L. A. Immunobiological preparations and prospects for their use in infektologii. М.: 2002. (in Russian)
- Otraslevoj Standart «Protokol vedenija bol'nyh. Disbakterioz kishechnika». OST 91500.11.0004-2003.Prikaz MZ RF № 231 ot 09.06.2003:70. (in Russian)
- Rakova E.B. The modern structure of the intestinal microbiota in the pediatric population of the Irkutsk region: Dis .... kand. biol. Science. Irkutsk, 2010. (in Russian)
- Rakova E.B., Popkova S.M., Nemchenko U.M i dr. Features microecological tsenotipov in children living in conditions of anthropogenic pressure// Gigiena i sanitarija, 2011; 4:22-26. (in Russian)
- Sidorenko A.V. Novik G.I., Akimov V.N. Using methods Genosystematics in the classification and identification of bacteria of the genus Bifidobacterium//Mikrobiologija. — 2008. — Т. 77 №3. — С. 293-302. (in Russian)
- Improving the methods of diagnosis of dysbiosis of the large intestine. Informacionnoe pis'mo. SPb.2002:15. (in Russian)
- Stefani D.V., Vel'tishhev Ju.E. Immunology and immunopathology of childhood (rukovodstvo dlja vrachej).- .M.:Medicina: 1996;384. (in Russian)
- Havkin A.I. Functional disorders of the gastrointestinal tract in infants. Posobie dlja vrachej.- М.:2001;16-17. (in Russian)
- Shenderov B.A. Medical microbial ecology and functional foods — М.: Grant, 1998. — Т. 1. — 286 с. — Т. 2. — 412 с., 2001. — Т. 3. —288 с. (in Russian)
14. Becker M.R., Paster B.J., Leys E.J. et al. Molecular analysis of bacterial species associated with childhood caries // J. Clin. Microbiol. 2002. 40. 1001–1009.
15. Collado M.C., Donat E., Ribes-Koninckx C. et al. Imbalances in faecal and duodenal Bifidobacterium species composition in active and non-active coeliac disease // BMC Microbiol. 2008. 8. PMC2635381.
16. Damman C.J., Miller S.I., Surawicz C.M., Zisman T.L. The microbiome and inflammatory bowel disease: is there a therapeutic role for fecal microbiota transplantation // Am. J. Gastroenterol. 2012. 107. (10). 1452–1459.
17. Kallus S.J., Brandt L.J. The intestinal microbiota and obesity // J. Clin. Gastroenterol. 2012. 46. (1). 16–24.
18. Lee Y.K. Effects of diet on gut microbiota profile and the implications for health and disease // Biosci. Microbiota Food Health. 2013. 32. (1). 1–12.
19. Matsuki T., Watanabe K., Fujimoto J. et al. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria // Appl. Environ. Microbiol. 2004. 70. (1). 167–173.
20. Paliy O. The golden age of molecular ecology // J. Phylogen. Evolution Biol. 2013. 1. e105.
21. Peterson J., Garges S., Giovanni M. et al. The NIH human microbiome project // Genome Res. 2009. 19. (12). 2317–2323.
22. Sharma R.‚ Young C., Mshvildadze M. et al. Intestinal microbiota: Does it play a role in diseases of the neonate? // NeoRev. 2009. 10. 166–179.
23. Weber T.K., Polanco I. Gastrointestinal microbiota and some children diseases: a review // Gastroenterol. Res. Pract. 2012. 2012. PMC3503313
24. Wu G.D., Chen J., Hoffmann C. et al. Linking long-term dietary patterns with gut microbial enterotypes // Science. 2011. 334. (6052). 105–108.
About Authors (Correspondence):
Nemchenko U.M. – junior researcher of the laboratory for microecology, e-mail: lav0467@mail.ru
Popkova S.M. – doctor of biological sciences, head of the laboratory for microecology, e-mail: smpopkova@gmail.com
Rakova E.B. – candidate of biological sciences, researcher of the laboratory for microecology, e-mail: lenova_@mail.ru
Rychkova L.V. – doctor of medical sciences, head of department of pediatrics
Serdyuk L.V. – candidate of biological sciences, researcher of the laboratory for microecology, e-mail: radugarose@yandex.ru
Ivanova E.I. – junior researcher of the laboratory for microecology, e-mail: ivanova.iem@gmail.com
Savel’kaeva M.V. – gastroenterologist, doctor of highest category
Dzhioev Yu.P. – candidate of biological sciences, senior researcher, e-mail: alanir07@mail.ru
Full Text