The effect of a lithium-containing composition on ethanol-induced changes in liver and brain within the experiments on mice
The work is aimed to assess the effect of lithium-containing composition on pathogenic pathway in liver and brain based on model of chronic alcohol abuse. Materials and methods. During the work we used nonlinear mice – males, weighing 25–30 g. For studies mice were randomly assigned to groups: first group was intact animals, mice from second and third groups received 40 % ethanol solution (3 g per 1 kg body weight) along with 5 % ethanol solution as a free drinking every day for 5 weeks; in addition starting from the third week of the experiment mice from the third group were administered suspension lithium-containing composition intragastrically. Starting from the third week of the experiment organs and blood samplings were taken every seven days for analysis. Results and discussion. Based on biochemical and histological analysis we determined that after lithium-containing composition administration it was observed reduction perineural and perivascular edemas in brain, reduction of total content of hyperchromic neurons and intercellular space reduction in liver among mice from the third intervention group in comparison with another negative control group. Proposed drug dosage form of lithium has a comprehensive protection effect on neurons and hepatic cells, it shows antitoxic property in case of chronic alcohol abuse.
About Authors (Correspondence):
Kotlyarova A.A. – postgraduate student, junior researcher of the laboratory of lymphoregulation, e-mai: firstname.lastname@example.org
Letyagin A.Yu. – doctor of medical sciences, leading researcher of the laboratory of lymphoregulation, professor, e-mail: email@example.com
Tolstikova T.G. – doctor of biological sciences, head of the laboratory of pharmacological investigation, professor
Bgatova N.P. – doctor of biological sciences, head of the laboratory ultrastructural researches, professor, e-mai: firstname.lastname@example.org
Rachkovskaya L.N. – candidate of chemical sciences, head of the laboratory of lymphoregulation, e-mai: email@example.com