Preventive intravenous injection of zymosan-treated neutrophils suppresses growth of B16 melanoma in mice liver and spleen
Author Affiliations1Research Institute of Fundamental and Clinical Immunology, 630099, Novosibirsk, Yadrintsevskaya str., 14
2Research Institute of Fundamental and Clinical Immunology, 630099, Novosibirsk, Yadrintsevskaya str., 14
3Research Institute for Experimental and Clinical and Medicine,630117, Novosibirsk, Timakov str., 2
4Research Institute of Fundamental and Clinical Immunology, 630099, Novosibirsk, Yadrintsevskaya str., 14
Abstract
We investigated the effect of activated ex vivo with zymosan and injected into tail vein neutrophils on the growth of B16 melanoma in mice liver and spleen. Our experiments showed that intravenous injection of zymosan-treated neutrophils 3 days before inoculation of B16 melanoma cell suspension results in a significant decrease of tumor nodule number in the liver (from 33.8 ± 10.9 to 0) and the weight gain of affected liver (from 612 ± 136 to 0 mg). The number of tumor nodules in the spleen dramatically decreased too (from 4.7 ± 0.6 to 0). The results indicate that intravenous injection of zymosan-treated neutrophils leads to a total suppression of B16 melanoma growth in mice liver and spleen.
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References
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About Authors (Correspondence):
Lyubimov G.Yu. – candidate of medical sciences, researcher, laboratory of immunopoiesis regulation, e-mail: glubimov@rambler.ru
Lyubimov A.G. – junior researcher, laboratory of immunopoiesis regulation, e-mail: glubimov@rambler.ru
Menshchikova E.B. – doctor of medical sciences, head of the laboratory for molecular mechanisms of free-radical processes, e-mail: lemen@centercem.ru
Kozlov V.A. – doctor of medical sciences, professor, academician of RAS, head of the laboratory of clinical immunopathology, е-mail: niiki01@online.nsk.su
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