Valve-containing xenovenous conduit: terra incognita or tabula rasa?
Abstract
The goal of this study was to evaluate bovine jugular vein as a potential material for original vascular conduit used in pediatric patients. Materials and methods. Endoscopic obtained bovine vein specimens were treated with either 6.25 % glutaraldehyde (GA), or 5 % ethylene glycol diglycidyl ether (DE), or both in different order. Histochemical, mechanical and calcium-binding properties of conserved material were evaluated. Interaction of the material and conserving solutions was also evaluated by means of IR-spectroscopy. Results. According to IR-spectroscopy, both GA and DE chemical bounds were identified in the wall of xeno-vein treated with GA+DE combination. The amount of conserving agent in specimens was determined by sequence of treatment. Independently of conservation method, all specimen demonstrated 1.7–2.7 higher (p < 0.05) mechanical strength in radial direction versus longitudinal direction. The greatest anisotropy of elasticity and mechanical strength was demonstrated in specimens treated with GA. Calcium-binding properties are not influenced by treatment method. The highest amount of calcium was found in GA-treated specimens and the lowest in DE-treated specimens (p = 0.00008). Specimens treated with DE+GA combination demonstrated medium quantities of calcium. Conclusion. Considering significant amount of collagen in conduit tissue, utilization of epoxy compounds is preferred for basic conservation, versus aldehyde compounds. This is because the former improves mechanical properties of the material and decreases its calcium binding potential.
Key words
Zhuravleva I.Yu. – doctor of medical sciences, professor, the head of the laboratory of bioprothesis, e-mail: i_zhuravleva@meshalkin.ru
Karpova E.V. – candidate of chemical science, senior researcher of the laboratory of physical methods, e-mail: karpovae@nioch.nsc.ru
Kuznetsova E.V. – junior researcher of the laboratory of bioprothesis, e-mail: ev_kuznetsova@meshalkin.ru
Yunoshev A.S. – candidate of physics and mathematics science, the head of the laboratory of high-speed processes, e-mail: yunoshev@hydro.nsc.ru
Korobeynikov A.A. – junior researcher of the laboratory of experimental surgery and morphology, e-mail: a_korobejnikov@meshalkin.ru
Timchenko T.P. – junior researcher of the laboratory of bioprothesis, e-mail: t_timchenko@meshalkin.ru
Nichay N.R. – junior researcher of the centre new technologies, cardiac surgery of department pediatric cardiac surgery, e-mail: n_nichay@meshalkin.ru
Soynov I.A. – junior researcher of the centre new technologies, cardiac surgery of department pediatric cardiac surgery, e-mail: i_sojnov@meshalkin.ru
Gorbatykh A.V. – candidate of medical sciences, junior researcher of the centre new technologies, cardiac surgery of department pediatric cardiac surgery, e-mail: a.gorbatykh@meshalkin.ru
