Immunological profile of patients with delayed bone tissue consolidation at long term after closed long bone injuries
Author Affiliations1Russian Scientific Center for Restorative Traumatology and Orthopaedics n.a. Ilizarov of Minzdrav of Russia 640014, Kurgan, Mariya Ulyanova str., 6
2Russian Scientific Center for Restorative Traumatology and Orthopaedics n.a. Ilizarov of Minzdrav of Russia 640014, Kurgan, Mariya Ulyanova str., 6
3Russian Scientific Center for Restorative Traumatology and Orthopaedics n.a. Ilizarov of Minzdrav of Russia 640014, Kurgan, Mariya Ulyanova str., 6
Abstract
Immunity parameters were studied in the period from 350 to 420 days after a closed long bone fracture treated with the Ilizarov method of transosseous osteosynthesis in patients with delayed bone union (n = 17) and fracture consolidation in optimal time periods (n = 20). The main parameters of cell and humoral immunity were within the limits of physiological norms in cases of normal reparative activity of the bone tissue. There was a tendency to the increase in IL-8 and Il-6 concentrations. The values of stimulated NBT test were increased. Delayed bone tissue consolidation was characterized by moderate activation of cell immunity along with increase in TNFα, decrease in CD14+HLA-DR expression, enhanced oxygen-dependent and oxygen-independent bactericidal activity of neutrophils, and neutrophil ability to form neutrophil extracellular traps.
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References
- Berdjugina O.V. Immunological prediction in traumatology and orthopedics. Yekaterinburg, 2009. 252 p. [In Russian].
- Chepeleva M.V., Karasev A.G., Samusenko D.V., Kuznetsova E.I. Immune status features in patients with delayed bone tissue consolidation after closed injury of long tubular bones // Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy = International journal of applied and fundamental research. 2014. (5-2). 87-91. [In Russian].
- Cheron A., Monneret G., Landelle C., Floccard B., Allaouchiche B. Low monocytic HLA-DR expression and risk of secondary infection // Ann. Fr. Anesth. Reanim. 2010. 29. (5). 368–376.
- Gumanenko E.K., Kozlova V.K. Polytrauma: traumatic illness, immune system dysfunction, modern treatment strategy. Moscow, 2008. 608 p. [In Russian].
- Kalinina N.M., Sosyukin A.E., Vologzhanin D.A. et al. Trauma: inflammation and immunity // Tsitokiny i vospalenie = Cytokines and inflammation. 2005. 4. (1). 28-35. [In Russian].
- Karieb S., Fox S.W. Suppression of T cell-induced osteoclast formation // Biochem. Biophys. Res. Commun. 2013. 436. (4). 619-24.
- Kotelnikov G.P., Trukhanova I.G. Traumatic disease. Moscow, 2009. 272 p. [In Russian].
- Kuznetsova E.I., Chepeleva M.V., Karasev A.G. Congenital immunity parameters in patients with delayed bone tissue union after a closed injury of long tubular bones // Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy = International journal of applied and fundamental research. 2015. (11-5). 652-654. [In Russian].
- Laboratory methods in clinical settings: Manual / Ed. V. V. Menshikov. Moscow: Meditsina, 1987. 365 p. [In Russian].
- Marusic A., Grcevic D., Katavic V. et al. Role of B lymphocytes in new bone formation // Lab. Invest. 2000. 80. (11). 1761-1774.
- Santolini E, West R., Giannoudis P. Risk factors for long bone fracture non-union: a stratification approach based on the level of the existing scientific evidence // Injury. 2015. 46. (8). 8–19.
- Savochkina A.Yu. Neutrophil extracellular traps: mechanisms of formation, methods of detection, biological role: abstract of thesis…doctor of medical sciences. Chelyabinsk, 2012. [In Russian].
- Urazgildeev P.Z., Kesyan G. A., Varfolomeev V.N. et al. Role of lipid peroxidation in the pathogenesis of false joints of long bones // Vestnik travmatologii i ortopedii imeni N.N. Priorova = Journal of Orthopaedics and traumatology named after N. N. Priorov. 2013. (2). 54–58.
About Authors (Correspondence):
Chepeleva M.V. – candidate of medical sciences, senior researcher of clinical laboratory of microbiology and immunology, e-mail: Vladi_2000@hotbox.ru
Kuznetsova E.I. – junior researcher of clinical laboratory of microbiology and immunology, e-mail: citoz@mail.ru
Karasev A.G. – doctor of medical sciences, associate professor, senior researcher of scientific-and-clinical laboratory of traumatology, e-mail: office@ilizarov.ru
