Influence of glyprolines PGP, RGP, PRPGP on DNA synthesis in the epithelium of tongue and duodenum of white mice under physiological conditions and on a model of gastroenteropathy
Author Affiliations1The Far-East State Medical University of Minzdrav of Russia, 680000, Khabarovsk, Muraviev-Amurskiy str., 35
2The Far-East State Medical University of Minzdrav of Russia, 680000, Khabarovsk, Muraviev-Amurskiy str., 35
Abstract
The purpose of study. Influence of glyprolines PGP, RGP, PRPGP on the DNA synthesis in the epithelium of the tongue and duodenum of white mice under physiological conditions and on the model indometacin gastropathy was studied. Material and methods. Male white mice were used in the experiment. Peptides were administered intraperitoneally at a dose of 1 mg / kg, indomethacin – 250 mg / kg. Proliferative activity was evaluated with 3Н-thymidine autoradiography (dosage 1 µgKu/g). We counted labeling nuclei index (LNI). Results and discussion. The fivefold peptides PGP, RGP, PRPGP introduction caused suppression of DNA synthesis in the surface epithelium of the tongue in physiological conditions. Peptide PRPGP reduces labeling nuclei index in the epithelium of duodenal. Intragastric administration of indomethacin caused the decrease in the proliferative activity of the surface epithelium of tongue and duodenum. Preliminary five times intraperitoneal injection of peptides PGP, RGP and PRPGP stimulates proliferative processes in the models of NSAID-gastropathy in the epithelium of the tongue and duodenum.
Key words
References
- Abdul R.M, Fernstrand A.M, Redegeld F.A. et al. The matrikine PGP as a potential biomarker in COPD. // Am. J. Physiol. Lung Cell. Mol. Physiol. 2015. 308. (11). L1095-L1101.
- Alekseyenko S.A., Timoshin S.S., Bolonyayeva N.A. et. al. Effect of the dalargine on reparative ability of the mucosa of the gastrointestinal tract at various gastroenterological diseases // Dal'nevostochnyy meditsinskiy zhurnal = Far East Medical Journal. 2010. (3). 15-18. [In Russian].
- Ashmarin I.P. Glyprolines composed regulatory tripeptides // Neyrokhimiya = Neurochemistry. 2007. (1). 5-7. [In Russian].
- Ashmarin I.P., Kamensky A.A., Lyapina L.A. et al. Glyprolines as independent regulators and regulators of other peptides // Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii = Problems of Biological, Medical and Pharmaceutical Chemistry. 2002. (1). 24–27. [In Russian].
- Bolonyayeva N.A., Zhivotova E.Yu., Fleyshman M.Yu. et al. Using of dalargine for the prevention and treatment of NSAID-gastropathy // Dal'nevostochnyy meditsinskiy zhurnal = Far East Medical Journal. 2005. (2). 28-30. [In Russian].
- Dudayev N.G. Clinico-morphological characteristic of enteropathy induced by non-steroidal anti-inflammatory drugs // Byulleten' medicinskikh internet-konferenciy = Bulletin of medical internet conferences. 2013. 3. (3). 577-579. [In Russian].
- Falaleyeva T.M., Samonina G.E., Beregovaya T.V. et al. Effect of glyprolines PGP, GP, and PG on homeostasis of gastric mucosa in rats with experimental ethanol-induced gastric ulcers // Bull. Exp. Biol. Med. 2010. 149. (6). 699–701.
- Fleishman M.Yu. Morphogenetic activity of dermorfine and its analogues in different cell populations: abstract of thesis…doctor of medical sciences. Vladivostok, 2010. [In Russian].
- Fleishman M.Yu., Kuznetsov A.V., Radivoz M.I. et al. Effect of sedatin, a synthetic dermorphin analog, on cell division in the corneal and lingual epithelia of rats // Bull. Exp. Biol. Med. 1996. 121. (6). 578–580.
- Fleishman M.Yu., Tolstenok I.V., Lebedko O.A. et al. Effect of glyprolines on DNA synthesis and free radical oxidation in mouse gastric mucosa under physiological conditions and during therapy with oral non-steroid anti-inflammatory drugs // Bull. Exp. Biol. Med. 2015. 159. (4). 502–504.
- Fleishman M.Yu., Zhivotova E.Yu., Lebed'ko O.A. et al. Protective effect of dermorphin analogue sedatin on indomethacin-induced injury to the gastric mucosa // Bull. Exp. Biol. Med. 2009. 148. (1). 60–63.
- Ozhegov E.V. Zhivotova E.Yu., Lebedko O.A. et al. Intensity of proliferative processes and degree of oxidative stress in the mucosa of the ileum in Crohn’s disease // Bull. Exp. Biol. Med. 2012. 152. (4). 420-423.
- Rochev D.L., Obergan T.Yu., Lyapina L.A. Assessing the impact of glyprolines PGPL and LPGP on fibrin polymerization processes and lipid profile in conditions in vivo // Tromboz, gemostaz i reologiya = Thrombosis, hemostasis and rheology. 2012. 52. (4). 45-49. [In Russian].
- Ryabov S.I., Zvyagintsev M.A., Dugin S.F. Bacterial colonization of indomethacin-induced ulcers of the small intestine of rat // Uspekhi sovremennogo yestestvoznaniya = Advances in current natural sciences. 2011. (3). 35-38. [In Russian].
- Shabalkin I.P., Grigor'eva E.Yu., Shabalkin P.I. et al. Reparation as a factor contributing to genetic variability of the biological system // Bull. Exp. Biol. Med. 2014. 158. (1). 170-175.
- Sostres C., Gargallo C.J., Lanas A. Nonsteroidal anti-inflammatory drugs and upper and lower gastrointestinal mucosal damage // Arthritis Res. Ther. 2013. 15. (Suppl. 3). S3.
- Storozhevykh T.P., Tukhbatova G.R., Senilova Ya.E. et al. Effect of semax and its Pro-Gly-Pro fragment on calcium homeostasis of neurons and their survival under conditions of glutamate toxicity // Bull. Exp. Biol. Med. 2007. 143. (5). 601–604.
- Thomas D.D., Ridnour L.A., Isenberg J.S. et al. The chemical biology of nitric oxide: implications in cellular signaling // Free Radic. Biol. Med. 2008. 45. (1). 18-31.
- Timoshin S.S., Fleischman M.Yu., Lebedko O.A. et al. Morphogenetic effects of dermorfine analogues in different cell populations // Dal'nevostochnyy meditsinskiy zhurnal = Far East Medical Journal. 2010. (3). 70-73. [In Russian].
- Vahrushev Ya.M., Zagrebina E.A. Enteropathy induced by non-steroidal anti-inflammatory drugs (pathogenesis, diagnosis, treatment) // Terapevticheskiy arkhiv = Therapeutic archive. 2012. 83. (5). 74-79. [In Russian].
- Vasilenko V.Kh., Tsodikov G.V. Minushkin O.N. Influence of some antirheumatic agents on the gastric mucosa // Klinicheskaya meditsina = Clinical medicine. 1971. (1). 3-9. [In Russian].
- Zhilyuk, V.I., Mamchur V.I. The role of endothelium in the mechanisms of neuroprotective effects of nootropic agents in conditions of hyperglycemia // Zhurnal natsional'noy akademii meditsinkikh nauk Ukraini = Journal of National Academy of Medical Sciences of Ukraine. 2013. 19. (2). 184-193. [In Russian].
- Zhivotova E.Yu., Lebed'ko O.A., Fleyshman M.Yu. et al. The participation of regulatory peptides in the regulation of tissue homeostasis gastric mucosa // Dal'nevostochnyy meditsinskiy zhurnal = Far East Medical Journal. 2013. (1). 101-105. [In Russian].
- Zhuykova S.E. Influence of some proline-containing peptides on gastric mucosal homeostasis in animal experiments: abstract of thesis…doctor of medical sciences. Moscow, 2003. [In Russian].
About Authors (Correspondence):
Fleyshman M.Yu. – doctor of medical sciences, assistant professor of the central scientific research laboratory, e-mail: marfl@yandex.ru
Tolstenok I.V. – applicant of the central scientific research laboratory, e-mail: toiv@bk.ru