The Siberian Scientific Medical Journal
 
 
№ 6 / 2017 / 10-15

STUDY OF RECOMBINANT APOLIPOPROTEIN A-I AS A CARRIER OF PLASMID DNA TO THE RAT HEPATOCYTES

Author Affiliations

Abstract

Purpose of the study was to obtain a modified variant of apolipoprotein A-I and studying its ability to transfer plasmid DNA into rat hepatocytes and comparison with the native form of the protein. Material and methods: gene cloning and production of recombinant proteins in E. coli, incubation of proteins in complex with plasmid pTagGFP2-C in vitro with rat hepatocytes, followed by cell analysis by flow cytometry. Results and discussion. A modified version of apo A-I (10 amino acid residue lysine added at the C-terminus of the protein) penetrated into the hepatocytes, specifically bound to the plasmid DNA, but did not transfect the plasmid into the cells.

Key words

apolipoprotein A-I, transfection, plasmid, hepatocyte
References
About Authors (Correspondence):

Polyakov L.M. – doctor of medical sciences, professor, head of the laboratory of medical biotechnology, e-mail: plm@niibch.ru

Full Text

Received: 09/01/2018