№ 6 / 2017 / 16-21

STUDY OF THE EFFECT OF LIPOSOMAL FORM OF DEXTRAZID ON THE EXPRESSION OF CATHEPSIN B AND D MACROPHAGES IN VITRO

Author: Neshchadim D.V.¹, Arkhipov S.A.², Shkurupiy V.A.³, Akhramenko E.S.⁴, Troitskiy A.V.⁵

Abstract

The effect of phosphatidylcholine liposomes (LP) with dextrazid (DZ) – antituberculosis agent (conjugate of oxidized dextran MW 60 kDa with isonicotinic acid hydrazide) on the expression of cathepsins B and D by peritoneal macrophages in mice was investigated in vitro. In the experiments we used LP of two sizes (0.15–0.20, 0.20–0.45 μm). Cultivation of MP in a culture medium containing LP with dextrazide (LP-DZ) of 0.15–0.20 μm and 0.20–0.45 μm for 1 day did not result in an increase in the number of macrophage expressing cathepsins B and D. The increase in the level of cathepsins B and D expression was observed with the use of LP-DZ of all sizes. This effect was more obvious when PL-DZ of 0.15–0.20 microns size were used. Probable the increase in the expression of macrophage cathepsins when LP-DZ were endocytosed by them was caused by the activation of macrophage-oxidized dextran which is the dextrazid component. The findings could be useful for understanding the mechanisms underlying the biological effects caused by liposomal forms of antiphthisic therapeutic agents mediated by MF which are the basic acceptors of LP-DZ and by the place of M.tuberculosis persistence.

Key words

in vitro, dextrazid, cathepsins, liposomes, macrophages, expression

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About Authors (Correspondence):

Neshchadim D.V. – candidate of biological sciences, researcher, e-mail: d.neshchadim@mail.ru

Full Text

snmzh_6-2017_neschadim_i_dr.pdf

Received: 10/01/2018