The Siberian Scientific Medical Journal
 
 
№ 4 / 2018 / 5-10
DOI 10.15372/SSMJ20180401

STUDY OF DNA DAMAGE BY NEW NORMOTHYMIC MEDICINAL ON THE BASIS OF THE COMPLEX OF LITHIUM CITRATE, POLYMETHYLSYLOXANE AND ALUMINUM OXIDE BY DNA COMET ASSAY IN VIVO

Author Affiliations

Abstract

The aim of the study was to study the effect of the normotimic drug based on a complex of lithium citrate, poly­methylsiloxane and alumina on DNA damage level (DNA comet test) in vivo by alkaline gel electrophoresis. It was shown that at 3 and 18 hours after the drug administration at the highest dose (2000 mg/kg) and therapeutic dose (400 mg/kg), the percentage of DNA in the tail in the cells of the bone marrow does not differ from the negative controls. That may indicate absence of possible carcinogenic action of the lithium-containing drug. On the other hand, the amount of DNA damage in other organs (kidney, liver, large intestine) increased with an exposure time of 3 hours after administration of the drug in a toxic dose. Although, the degree of this effect expression was not high. When the dose was reduced (400 mg/kg) and the duration of the experiment was increased (up to 18 hours), this effect was not detected. That indicates the ability of the drug to enhance DNA damage repair. Given that lithium preparations have a pronounced toxic effect on kidneys, gastrointestinal tract, liver, the increase in the percentage of DNA in the tail in these organs can be a consequence of the cytostatic action of the drug.

Key words

medicinal product, a complex of lithium citrate, polymethylsiloxane and aluminum oxide, DNA damage, DNA comet asay
References
About Authors (Correspondence):

Shurlygina A.V. – doctor of medical sciences, professor, leading researcher, e-mail: anna_v_s@mail.ru

Full Text

Received: 29/08/2018
Accepted: 29/08/2018