The Siberian Scientific Medical Journal
 
 
№ 1 / 2012 / 38-42

Features of interaction of apolipoprotein А-I and its complexes with tetrahydrocortisol with prokaryotic DNA

Author Affiliations

Abstract

The influence of apolipoprotein А-I on secondary structure of prokaryotic DNA of a plasmid pBluescript has been investigated with the use of small angle X-ray scattering method and an electrophoresis in agarose gel. It has been shown that transition of the super braided form of a plasmid in ring under the influence of apolipoprotein А-I and its complexes with tetrahydrocortisol occurs at the expense of rupture of hydrogen bonds between the nitrogenous bases complementary DNA threads. Change of secondary structure of DNA of a plasmid occurred also at its incubation to apolipoprotein А-I without a hormone. The participation of apolipoprotein А-I in such processes of cell metabolism as replication and transcription has been discussed.

Key words

apolipoprotein А-I, small angle X-ray scattering, plasmid, , , ,, super braided and ring DNA, tetrahydrocortisol
References
About Authors (Correspondence):

Panin L.E. – academician of RAMS, director, e-mail:ibch@soramn.ru

Kovalenko G.A. – candidate of biological sciences, senior researcher of laboratory of molecular mechanisms of intercellular interactions, e-mail: ibch@soramn.ru

Polyakov L.M. – doctor of medical sciences, professor, head of laboratory for medical biotechnology,
e-mail: plm@soramn.ru

Tuzikov F.V.doctor of biological sciences, senior researcher of laboratory for medical biotechnology,
e-mail: tuzikov@catalysis.ru

Tuzikova N.A.researcher of laboratory medical biotechnology, e-mail: tuz54@mail.ru

Knyazev R.A. – candidate of biological sciences, senior researcher of laboratory of molecular mechanisms
of intercellular interactions, e-mail: knjazev@soramn.ru

Full Text

Received: 09/02/2015