Features of interaction of apolipoprotein А-I and its complexes with tetrahydrocortisol with prokaryotic DNA

The influence of apolipoprotein А-I on secondary structure of prokaryotic DNA of a plasmid pBluescript has been investigated with the use of small angle X-ray scattering method and an electrophoresis in agarose gel. It has been shown that transition of the super braided form of a plasmid in ring under the influence of apolipoprotein А-I and its complexes with tetrahydrocortisol occurs at the expense of rupture of hydrogen bonds between the nitrogenous bases complementary DNA threads. Change of secondary structure of DNA of a plasmid occurred also at its incubation to apolipoprotein А-I without a hormone. The participation of apolipoprotein А-I in such processes of cell metabolism as replication and transcription has been discussed.

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Knyazev R.A. – candidate of biological sciences, senior researcher of laboratory of molecular mechanisms
of intercellular interactions, e-mail: knjazev@soramn.ru