EFFECTS OF PEPTIDE SELANK ON OXIDATIVE STRESS IN THE BRAIN AND THIN INTESTINE OF WHITE RATS ON EXPERIMENTAL MODEL OF TRAUMATIC BRAIN INJURY

Author: Fleyshman M.Yu.¹, Tolstenok I.V.², Innokent’ev A.A.³

Abstract

Effects of glyproline-containing peptide Selank on oxidative stress parameters in the brain and thin intestine were studied. Material and methods. Traumatic brain injury (TBI) was induced in rats by a shock model (weight drop method). Three groups were formed: «Intact control» (injection of sodium chloride isotonic solution), «control with TBI» (TBI model + injection of sodium chloride isotonic solution), «“Selank” with TBI» (TBI model + injection of peptide solution at a dose of 0.1 mg/kg body weight). All solutions were administered intraperitoneally in a volume of 0.5 ml for 5 days, in groups 2 and 3 the first injection was introduced in 3 hours after TBI. The evaluation of free radical oxidation processes in animal tissues was performed by chemiluminescence method. Analysis was performed in homogenates of fresh biomaterial, the results, obtained in millivolts, were calculated per 1 gram of wet tissue and were presented in relative units. Results. Post-traumatic effects in brain tissue have led to the intensification of free radical oxidation processes. Hyperproduction of free radicals, lipid hydroperoxides was observed, the rate of peroxide radical formation increased. In homogenates of white rat small intestine the stress effect did not cause a change in chemiluminescence parameters. Peptide «Selank» administration at a dose of 0.1 mg/kg after traumatic stress led to a significant decrease in all chemiluminescence indices in the tissues.

Key words

traumatic brain injury, glyprolines, peptide «Selank», chemiluminescence, oxidative stress

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About Authors (Correspondence):

Fleyshman M.Yu. – doctor of medical sciences, chief researcher of the central research laboratory, e-mail: marfl@yandex.ru

Full Text

snmzh_2-2019_fleyshman_46-51.pdf

Received: 24/04/2019

Accepted: 24/04/2019