STUDYING THE POSSIBLE MUTAGENIC PROPERTIES OF NEW MEDICINE ON THE BASIS OF COMPLEX LITHIUM CITRATE, ALUMINUM OXIDE AND POLYMETHILSILOXANE

Author: Konenkov V.I.¹, Korolev M.A.², Churin A.A.³, Voronova O.L.⁴, Neupokoeva O.V.⁵, Rachkovskaya L.N.⁶, Shurlygina A.V.⁷, Robinson M.V⁸, Kotlyarova A.A.⁹, Popova T.V.¹⁰, Rachkovskiy E.E.¹¹, Madonov P.G.¹², Letyagin A.Yu.¹³

Author Affiliations

¹Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

²Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

³Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center 634028, Tomsk, Lenin av., 3

⁴Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center 634028, Tomsk, Lenin av., 3

⁵Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center 634028, Tomsk, Lenin av., 3

⁶Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

⁷Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

⁸Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

⁹Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

¹⁰Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

¹¹Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

¹²Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

¹³Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics of SB RAS 630060, Novosibirsk, Timakov str., 2

Abstract

Aim of the study was to investigate the possible mutagenic properties of a new drug based on a lithium-containing substance – a complex of lithium citrate, polymethylsiloxane and aluminum oxide. Material and methods. Methods for testing mutagenicity using chromosomal aberrations in the bone marrow cells of CBA mice and somatic recombination in Drosophila melanogaster were used. Results. It was shown that a single intragastric administration of drug at a dose of 5000 mg/kg and a fivefold course of administration at a dose of 400 mg/kg to CBA mice did not increase the level of cytogenetic disorders in bone marrow cells. The study of the lithium complex drug in a somatic mosaicism test revealed that the preparation at a dose of 2000 mg/kg does not increase the frequency of mutations in Drosophila melanogaster. Conclusion. A single intragastric administration of the studied drug at a dose of 5000 mg/kg and its course administration (400 mg/kg × 5) do not increase the level of cytogenetic disorders in the bone marrow cells of CBA mice. In the somatic recombination (mosaicism) test system on D. melanogaster, no increase in the appearance of mutant setae and spots on the body and head was observed when using yellow and singed markers. The results of the study indicate that the studied drug does not have mutagenic properties.

Key words

mutagenicity, drug based on complex lithium citrate, polymethylsiloxane and alumina oxide, Drosophila melanogaster, somatic recombination, CBA mice, preclinical research

References

About Authors (Correspondence):

Shurlygina A.V., e-mail: anna_v_s@mail.ru

Full Text

snmzh_5-2019_konenkov_i_dr.pdf

Received: 30/10/2019

Accepted: 30/10/2019