DOI 10.15372/SSMJ20190607
ANALYSIS OF HAPLOTYPES OF CAT, TLR4, AND IL10 GENES IN BRONCHIAL ASTHMA PATIENTS COMORBID WITH ARTERIAL HYPERTENSION
Author: Bragina E.Yu.1, Goncharova I.A.2, Freidin M.B.3, Zhalsanova I.Zh.4, Gomboeva D.E.5, Nemerov E.V.6, Puzyrev V.P.7
Author Affiliations
1Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10
2Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10
3Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10
4Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10
5Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10
6Siberian State Medical University of Minzdrav of Russia 634050, Tomsk, Moskovsky path, 2
7Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS 634050, Tomsk, Naberezhnaya reki Ushayky str., 10; Siberian State Medical University of Minzdrav of Russia 634050, Tomsk, Moskovsky path, 2
Abstract
Co-occurrence of cardiovascular diseases is significantly common among patients with bronchial asthma. Genetic factors can have a significant effect on the development of hypertension in patients with asthma. Objective of the study was to investigate the associations of polymorphic variants relating to quantitative changes in the expression profile (eQTL) of the CAT, TLR4, and IL10 genes with the development of bronchial asthma co-morbid with arterial hypertension. Material and methods. Genotyping of 48 eQTL SNPs of the CAT, TLR4, and IL10 genes was performed using MALDI-TOF mass spectrometry in patients with «isolated» asthma (n = 145) and arterial hypertension (n = 144) and their combination (n = 146), as well as in the control group of healthy individuals (n = 152). Using logistic regression, an analysis of the associations of haplotypes with the studied diseases was carried out. Results. An association of bronchial asthma in combination with arterial hypertension with haplotypes formed by eQTL SNPs of the CAT and TLR4 genes was established. The spectrum of haplotypes associated with comorbidity of asthma and hypertension differs from the haplotypes associated with “isolated” asthma. Conclusion. The molecular base of asthma and hypertension comorbidity can be associated with variants that control the expression of TLR4 and CAT genes.
Key words
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About Authors (Correspondence):
Bragina E.Yu., e-mail: elena.bragina72@gmail.com
Full Text
Received: 26/12/2019
Accepted: 26/12/2019
