The Siberian Scientific Medical Journal
 
 
№ 6 / 2012 / 15-21

The role of intracellular proteinases in regulation of the HIF-1α, NF-κВ transcription factors expression and VEGF at lymphatic cancer spread of squamous cell carcinoma of head and neck

Author Affiliations

Abstract

Aim of the study was to determine the NF-kB, HIF-1a и VEGF expression, their characteristics in lymph node metastasis development of squamous cell carcinoma of head and neck. Material and methods: 66 patients with squamous cell carcinoma of head and neck (Т2–3N0–2). Transcription factors and VEGF expression was measured by ELISA kits. Proteasome and calpain activity was determined using specific fluorogenic substrate. Proteasome subunits composition was measured by Western Blot Analysis. Results and discussion. The obtained data revealed the association of lymph node metastasis development with NF-kB р50 expression and LMP2 proteasome subunits content.

Key words

calpain, squamous cell carcinoma of head and neck., proteasome, transcription factors NF-B and HIF-1, VEGF
References
About Authors (Correspondence):

Choinzonov E.L. – doctor of medical sciences, professor, academician of RAMS, director, e-mail: nii@oncology.tomsk.ru

Spirina L.V. – candidate of medical sciences, senior researcher of laboratory of tumor biology, e-mail: spirinalv@oncology.tomsk.ru

Kondakova I.V. – doctor of medical sciences, professor, head of laboratory of tumor biochemistry, e-mail: kondakova@oncology.tomsk.ru

Chizhevskaya S.Yu. – candidate of medical sciences, senior researcher of head and neck cancer department, e-mail: nii@oncology.tomsk.ru

Shishkin D.A. – candidate of medical sciences, senior researcher of head and neck cancer department, e-mail: niio@sibmail.com

Kulbakin D.E. – junior researcher of head and neck cancer department, e-mail: nii@oncology.tomsk.ru

Full Text

Received: 09/02/2015