The Siberian Scientific Medical Journal
 
 
№ 3 / 2014 / 31-36

Ultrastructural analysis of tear fluid in patients suffering from diabetic retinopathy

Author Affiliations

Abstract

AIM: to determine the ultrastructural characteristics of the tear fluid of patients with diabetic retinopathy. Materials and methods: tear fluid was collected from patients and healthy individuals, and were examined by negative staining in electron microscope Jem 1400 (JEOL, Japan). Exosome fraction also was prepared and studied. Results and discussion: the suitability of tear fluid for ultrastructural analysis of its components and isolation of exosomes was shown. Increased concentration of exosomes, changes in their morphology, as well as changes in the content of other microparticles and macromolecular aggregates in the tear fluid of patients with diabetic retinopathy was found.

Key words

diabetic retinopathy, microparticles, lacrimal fluid, electron microscopy
References
About Authors (Correspondence):

Grigor’eva A.E. – postgraduate student, e-mail: feabelit@mail.ru

Eremina A.V. – ophthalmologist, e-mail: sci@mntk.nsk.ru

Tamkovich S.N. – candidate of biological sciences, е-mail: s.tamk@niboch.nsc.ru

Chernykh V.V. – doctor of medical sciences, professor, director, e-mail: rimma@mntk.nsk.ru

Vlassov V.V. – academician of RAS, director, е-mail: vvv@niboch.nsc.ru

Ryabchikova E.I. – doctor of biological sciences, head of microscopy group, e-mail: lenryab@yandex.ru

Full Text

Received: 09/02/2015