The Siberian Scientific Medical Journal
 
 
№ 4 / 2014 / 42-47

Evaluation of level of cytokines produced by peripheral blood mononuclear cells of patients with chronic heart failure after mobilization of bone marrow stem cells with G-CSF

Author Affiliations

Abstract

The influence of G-CSF introduction on cytokine production by peripheral blood mononuclear cells after their enrichment with the bone marrow stem cells has been investigated. It is shown that mononuclear cells of patients with chronic heart failure in a 48-hour culture in vitro produce a wide range of cytokines with regulatory effect both in spontaneous conditions and when stimulated by mitogens. The findings suggest the feasibility of using mononuclear cells after mobilization with G-CSF to stimulate angiogenesis and repаrativе processes in the myocardium.

 

Key words

granulocyte colony-stimulating factor, coronary heart disease, stem cells, cytokines
References
About Authors (Correspondence):

Poveshchenko O.V. – candidate of medical sciences, head of the laboratory of lymphotropic therapy and lymphodiagnostic, e-mail: poveschenkoov@yandex.ru

Kim I.I. – researcher of laboratory of lymphotropic therapy and lymphodiagnostic, e-mail: kii5@yandex.ru

Shevchenko A.V. – candidate of biologic sciences, senior researcher of laboratory for clinical immunogenetics, e-mail: shalla64@mail.ru

Pokushalov E.A. – doctor of medical sciences, vice-director, e-mail: cpsc@nricp.ru

Romanov A.B. – candidate of medical sciences, surgeon of the centre of surgical arrhythmology, e-mail: aromanov1981@rambler.ru

Bondarenko N.A. – postgraduate student of laboratory of lymphotropic therapy and lympho diagnostic, e-mail: bond802888@yandex.ru

Lykov A.P. – candidate of medical sciences, leading researcher of laboratory of lymphotropic therapy and lympho diagnostic, e-mail: lykovalex@freemail.ru

Poveshchenko A.F. – doctor of medical sciences, head of the laboratory of physiology of the protective system, e-mail: poveshchenkoa200@mail.ru

Karaskov А.М. – academician of RAS, doctor of medical sciences, director

Konenkov V.I. – academician of RAS, doctor of medical sciences, director, e-mail: lymphology@soramn.ru

Full Text

Received: 08/02/2015